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  • Director of Center
  • Director of Department
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© Giulia Manina, Institut Pasteur
Mycobacterium tuberculosis dual fluorescent reporter of metabolic activity. Green (active bacilli) and red fluorescence (quiescent bacilli) are merged, 100X magnification.
Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
07
Sep 2015
Status
Ongoing
Members
3
Structures
3
Instituts
1

About

Fluorescent live cell imaging and single-cell analysis provide a clear glimpse into bacterial individuality. Discrete subpopulations can be separated either by conventional FACS or droplet sorting, and enriched subsets can be subjected to global downstream analysis, such as RNA-sequencing. This approach aims to dig out new coding and/or non-coding sequences, particularly abundant during states of low metabolic activity, as hallmarks of quiescence.