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© Giulia Manina, Institut Pasteur
Mycobacterium tuberculosis dual fluorescent reporter of metabolic activity. Green (active bacilli) and red fluorescence (quiescent bacilli) are merged, 100X magnification.
Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
07
Sep 2015
Status
Ongoing
Members
2
Structures
3
Instituts
1

About

One of the significant drivers of phenotypic heterogeneity is noise in gene expression, which in turn is greatly influenced by fluctuations in the number of RNA molecules. Subcellular visualization of RNA turnover and quality control can aid in gaining new insights into gene expression regulation and cell behavior under different growth and stress conditions. This can be effectively addressed using short-lived and aptamer-based fluorescent reporters in conjunction with time-lapse microfluidic microscopy.

Fundings