Seminar of the Structural Biology and Chemistry department
Tuesday 15 January 2019 at 2.00 pm
Salle AUDITORIUM CENTRE F. JACOB – CFJ RdC 17c
Dr. Mélanie ETHEVE-QUELQUEJEU
Université Paris Descartes
Modified RNAs as Molecular Tools for Structural and Functional Studies of aminoacyl transferases and methyltransferases enzymes.
Abstract. The role of RNAs in cellular processes is one of the most dynamic areas in Biology. In addition to their key functions in protein synthesis, new RNA activities are constantly discovered. To study these different biological processes, in particular, the enzymes that interact with these RNAs, we have developed synthetic methodologies to obtain stable RNAs, reactive RNAs or peptidyl-conjugates. The use of nucleoside and nucleotides chemistry, solid support synthesis, enzymatic reactions or post-functionalization methods allowed us to obtain a large variety of modified RNAs, which will be presented here. These RNA analogs were used for the study of Fem aminoacyl transferases, a tRNA-dependent enzyme that catalyze an essential step in peptidoglycan synthesis of the bacterial wall and for the study of m6A-RNA methyltransferases.
Contact : Marcel Hollenstein
Groupe à 5 ans Chimie Bioorganique des Acides Nucléiques