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© Research
Scientific Fields
Diseases
Organisms
Applications
Technique
Date
22
Jan 2019
Time
14:00:00
28 Rue du Dr Roux, Paris, France
Address
Building: François Jacob Room: Auditorium François Jacob
Location
2019-01-22 14:00:00 2019-01-22 18:00:00 Europe/Paris Seminar Structural Biochemistry and Chemistry Department: Dr. Shana Sturla Seminar Structural Biochemistry and Chemistry Department Tuesday 22 january 2019 at 2.00 pm Salle AUDITORIUM CENTRE F. JACOB – CFJ RdC 17c  Dr. Shana Sturla  ETH Zurich   DNA adduct molecular probes: From mechanisms […] 28 Rue du Dr Roux, Paris, France Marcel Hollenstein marcel.hollenstein@pasteur.fr

About

Seminar Structural Biochemistry and Chemistry Department

Tuesday 22 january 2019 at 2.00 pm

Salle AUDITORIUM CENTRE F. JACOB – CFJ RdC 17c

 Dr. Shana Sturla

 ETH Zurich

 

DNA adduct molecular probes: From mechanisms of mutagenesis to adduct mapping and amplification

Carcinogenesis can be associated with an ordered progression of acquired mutations, in some cases with significant portions corresponding to point mutations in critical cell growth regulating genes.  The biological generation of chemical alkylating agents or reactive oxidation species from endogenous metabolic processes and chemical exposures can alter the structures of DNA bases in genomic DNA.  Using chemistry-based approaches, we have characterized the influence of DNA alkylation on polymerase-mediated synthesis of DNA, including the use of novel synthetic damage-directed nucleoside probes to understand chemical factors that influence the fidelity of DNA synthesis.  Additionally, with the aim of amplifying damaged DNA for diagnostic purposes, we have demonstrated the efficient and specific incorporation of synthetic nucleotides opposite a DNA adduct but not template bases as a marker for specific positions of alkylation in DNA.  Finally, we have demonstrated the use of alkynylated nucloetides for click-labeling of DNA at positions of damage, allowing us to map the frequency of modifications as a function of local sequence characteristics and broad genomic features.  These results provide new insight into how base misincorporations lead to mutations, together with chemical tools for locating DNA damage within sequence contexts on entire genomes.

Contact : Marcel Hollenstein

Groupe à 5 ans Chimie bioorganique des acides nucléiques

Marcel.hollenstein@pasteur.fr

 

Location

Building: François Jacob
Room: Auditorium François Jacob
Address: 28 Rue du Dr Roux, Paris, France