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  • Deputy Director of Center
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  • Deputy Head of Facility
  • Director of Center
  • Director of Department
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Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
07
Feb 2022
Ending Date
18
Feb 2022
Time
09:00:00
28 Rue du Docteur Roux, Paris, France
Address
2022-02-07 09:00:00 2022-02-18 18:00:00 Europe/Paris Multiple roles of RNAs A practical course on RNA methods RNA research is at the basis of our current understanding of gene expression regulation and was crucial for the discovery of tools such as siRNA and CRISPR/Cas9. RNA […] 28 Rue du Docteur Roux, Paris, France Gwenaël Badis-Breard gwenael.badis-breard@pasteur.fr
A practical course on RNA methods

RNA research is at the basis of our current understanding of gene expression regulation and was crucial for the discovery of tools such as siRNA and CRISPR/Cas9. RNA degradation mechanisms are essential for the defense of eukaryotic cells against many viruses, and the studies of molecular mechanisms by which viruses fight back are important to understand host-pathogen interactions.

One of the best studied RNA degradation pathways, which limits viral replication and shapes the transcriptome by “cleaning” aberrant non-coding RNAs is the “Nonsense mRNA Mediated Decay” (NMD). NMD monitors the capacity of mRNAs to be adequately translated into full-length proteins. NMD degrades mRNAs with premature termination codons (PTCs), a diverse class of RNA molecules that are by-products in the natural process of RNA synthesis because RNA splicing can be inefficient or RNA polymerase can often start transcription after the position of the start codon (e.g. Malabat et al., eLife, 2015).

During the course, the students will become familiar with three major experimental strategies for RNA quantification: RNA sequencing, RT-qPCR and Northern blotting. These methods will be used to estimate how NMD affects cellular transcripts and to learn how to look at the data beyond the obtained numbers. Interpretation of the results will include a brief introduction about using command-line tools and R for data analysis and interpretation.

Attendants

While this practical course is includes mostly master degree (M2) students, it is open to young scientists who begin working on RNA. Candidates should be familiar with molecular biology bench work and will need basic bioinformatic skills.

More information here.

Directors: Gwenaël Badis-Breard and Claire Torchet

Head of studies: Cosmin Saveanu

Partners

Location

Location: 28 Rue du Docteur Roux, Paris, France