Timeline

Alexandre Chenal

Group Leader

20 Nov 2023

publication

B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles.

25 May 2023

event

The 29th Meeting on Toxinology is now being organized!

23 Mar 2023

publication

Gallocin A, an Atypical Two-Peptide Bacteriocin with Intramolecular Disulfide Bonds Required for Activity.

08 Oct 2022

publication

A Robust and Sensitive Spectrophotometric Assay for the Enzymatic Activity of Bacterial Adenylate Cyclase Toxins.

08 Sep 2022

event

28th Meeting on Toxinology (RT28) – November 28th-29th, 2022

09 Aug 2022

publication

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium.

01 May 2021

publication

A High-Affinity Calmodulin-Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells.

15 Oct 2020

publication

Development of Conformational Antibodies to Detect Bcl-xL’s Amyloid Aggregates in Metal-Induced Apoptotic Neuroblastoma Cells.

18 Aug 2020

publication

Dissecting the Structural and Chemical Determinants of the “Open-to-Closed” Motion in the Mannosyltransferase PimA from Mycobacteria.

02 Apr 2020

publication

Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum.

01 Jan 2020

publication

Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins.

01 Jan 2020

publication

Functional and structural consequences of epithelial cell invasion by Bordetella pertussis adenylate cyclase toxin.

20 Dec 2019

event

! CANCELED ! 1st Italian-French-German Joint Meeting on Toxinology

03 Sep 2019

event

PhD defense of Alexis Voegele: Study of the translocation mechanism of the CyaA toxin from Bordetella pertussis

21 Jun 2019

publication

Post-translational acylation controls the folding and functions of the CyaA RTX toxin.

13 Feb 2019

publication

The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro.

03 Jan 2019

event

Department of Structural Biology and Chemistry Department: Paolo de Los Rios, Non-equilibrium protein folding (and unfolding) in the cell.

01 Nov 2018

publication

Translocation and calmodulin-activation of the adenylate cyclase toxin (CyaA) of Bordetella pertussis.

20 Jul 2018

publication

Bioengineering of Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy

14 Nov 2017

publication

Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins

14 Nov 2017

publication

Membrane-Active Properties of an Amphitropic Peptide from the CyaA Toxin Translocation Region

28 Sep 2017

publication

Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution (Review)

26 Jul 2017

publication

Exposure to Bordetella pertussis adenylate cyclase toxin affects integrin-mediated adhesion and mechanics in alveolar epithelial cells

10 Feb 2017

publication

Stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, CyaA, from Bordetella pertussis

27 Jan 2017

event

HDX-MS/NMR DAY

01 Jan 2017

publication

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis.

18 Aug 2016

publication

From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide

13 Jul 2016

publication

MEMHDX: an interactive tool to expedite the statistical validation and visualization of large HDX-MS datasets.

09 May 2016

publication

Molecular Basis of Membrane Association by the Phosphatidylinositol Mannosyltransferase PimA Enzyme from Mycobacteria.

25 Mar 2016

publication

The Tip of the Four N-Terminal α-Helices of Clostridium sordellii Lethal Toxin Contains the Interaction Site with Membrane Phosphatidylserine Facilitating Small GTPases Glucosylation

20 Nov 2015

project

Biophysical characterization of the translocation process of Bordetella pertussis adenylate cyclase toxin CyaA

20 Nov 2015

publication

Anchoring Sites of Fibrillogenic Peptide Hormone Somatostatin-14 on Plasmonic Nanoparticles.

18 Nov 2015

project

Molecular crowding

16 Sep 2015

publication

Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion.

23 Aug 2015

publication

Structure and function of RTX toxins.

17 Aug 2015

project

CyaA secretion, folding and translocation across membrane

04 Aug 2015

publication

Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins

21 Jul 2015

project

Development of a new statistical tool for the analysis of large HDX-MS data sets

16 Jul 2015

team

BiophysiCyaA

13 May 2015

publication

The catalytic domains of Clostridium sordellii lethal toxin and related large clostridial glucosylating toxins specifically recognize the negatively charged phospholipids phosphatidylserine and phosphatidic acid

11 May 2015

team

Biochemistry of Macromolecular Interactions

31 Dec 2014

publication

Disorder-to-order transition in the CyaA toxin RTX domain: implications for toxin secretion

17 Nov 2014

publication

Secondary structure reshuffling modulates glycosyltransferase function at the membrane.

17 Sep 2014

publication

Calcium, acylation, and molecular confinement favor folding of Bordetella pertussis adenylate cyclase CyaA toxin into a monomeric and cytotoxic form

25 Jul 2014

publication

Allosteric activation of Bordetella pertussis adenylyl cyclase by calmodulin: molecular dynamics and mutagenesis studies

02 Dec 2013

publication

Bordetella pertussis adenylate cyclase toxin translocation across a tethered lipid bilayer

24 Sep 2013

publication

Characterization of a membrane-active peptide from the Bordetella pertussis CyaA toxin

31 Jul 2013

publication

Molecular crowding stabilizes both the intrinsically disordered calcium-free state and the folded calcium-bound state of a repeat in toxin (RTX) protein

12 Jan 2012

publication

Identification of a region that assists membrane insertion and translocation of the catalytic domain of Bordetella pertussis CyaA toxin

01 Jan 2012

publication

Mean net charge of intrinsically disordered proteins: experimental determination of protein valence by electrophoretic mobility measurements

01 Jan 2012

publication

Estimation of intrinsically disordered protein shape and time-averaged apparent hydration in native conditions by a combination of hydrodynamic methods

19 Nov 2011

publication

Amyloid fibrils formed by the programmed cell death regulator Bcl-xL

15 Mar 2011

publication

Calcium-induced folding of intrinsically disordered repeat-in-toxin (RTX) motifs via changes of protein charges and oligomerization states

01 Dec 2010

publication

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin

22 Jan 2010

publication

Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin

19 Jan 2010

publication

Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin

24 Jul 2009

publication

Clostridium septicum alpha-toxin forms pores and induces rapid cell necrosis

01 Jul 2009

publication

Deciphering membrane insertion of the diphtheria toxin T domain by specular neutron reflectometry and solid-state NMR spectroscopy

13 May 2009

publication

Side chain resonances in static oriented proton-decoupled 15N solid-state NMR spectra of membrane proteins

23 Mar 2009

publication

Cellular functions and X-ray structure of anthrolysin O, a cholesterol-dependent cytolysin secreted by Bacillus anthracis

17 Nov 2008

publication

RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion

07 Aug 2008

publication

Membrane Interaction of botulinum neurotoxin A translocation (T) domain. The belt region is a regulatory loop for membrane interaction

21 Jun 2007

publication

Concerted protonation of key histidines triggers membrane interaction of the diphtheria toxin T domain

15 Mar 2007

publication

Type III secretion effectors of the IpaH family are E3 ubiquitin ligases

13 Feb 2007

publication

Defining the interacting regions between apomyoglobin and lipid membrane by hydrogen/deuterium exchange coupled to mass spectrometry

24 Jan 2007

publication

Behavior of the N-terminal helices of the diphtheria toxin T domain during the successive steps of membrane interaction

22 Jan 2007

publication

Interactions of apomyoglobin with membranes: mechanisms and effects on heme uptake

20 Apr 2006

publication

Structural and functional characterization of an essential RTX subdomain of Bordetella pertussis adenylate cyclase toxin

17 Jun 2005

publication

Conformational states and thermodynamics of alpha-lactalbumin bound to membranes: a case study of the effects of pH, calcium, lipid membrane curvature and charge

16 Feb 2005

publication

Characterization of wild-type recombinant Bet v 1a as a candidate vaccine against birch pollen allergy

30 Sep 2004

publication

Alteration of the tertiary structure of the major bee venom allergen Api m 1 by multiple mutations is concomitant with low IgE reactivity

13 Aug 2004

publication

Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin A is essential for the generation of protective antibodies

13 Aug 2004

publication

Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin A is essential for the generation of protective antibodies

01 Jan 2003

publication

Anchoring cytokines to tumor cells for the preparation of anticancer vaccines without gene transfection in mice

06 Sep 2002

publication

Clostridium perfringens iota toxin. Mapping of the Ia domain involved in docking with Ib and cellular internalization

21 Aug 2002

publication

Membrane protein insertion regulated by bringing electrostatic and hydrophobic interactions into play. A case study with the translocation domain of diphtheria toxin

01 May 2002

publication

Does fusion of domains from unrelated proteins affect their folding pathways and the structural changes involved in their function? A case study with the diphtheria toxin T domain

01 Jun 2001

publication

Prolonged display or rapid internalization of the IgG-binding protein ZZ anchored to the surface of cells using the diphtheria toxin T domain

01 Jan 2001

publication

A little guide for advanced search:

B2LiVe, a label-free 1D-NMR method to quantify the binding of amphitropic peptides or proteins to membrane vesicles.

The 29th Meeting on Toxinology is now being organized!

Gallocin A, an Atypical Two-Peptide Bacteriocin with Intramolecular Disulfide Bonds Required for Activity.

A Robust and Sensitive Spectrophotometric Assay for the Enzymatic Activity of Bacterial Adenylate Cyclase Toxins.

28th Meeting on Toxinology (RT28) – November 28th-29th, 2022

Dynamics and structural changes of calmodulin upon interaction with the antagonist calmidazolium.

A High-Affinity Calmodulin-Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells.

Development of Conformational Antibodies to Detect Bcl-xL’s Amyloid Aggregates in Metal-Induced Apoptotic Neuroblastoma Cells.

Dissecting the Structural and Chemical Determinants of the “Open-to-Closed” Motion in the Mannosyltransferase PimA from Mycobacteria.

Essential dynamic interdependence of FtsZ and SepF for Z-ring and septum formation in Corynebacterium glutamicum.

Hydrogen/Deuterium Exchange Mass Spectrometry for the Structural Analysis of Detergent-Solubilized Membrane Proteins.

Functional and structural consequences of epithelial cell invasion by Bordetella pertussis adenylate cyclase toxin.

! CANCELED ! 1st Italian-French-German Joint Meeting on Toxinology

PhD defense of Alexis Voegele: Study of the translocation mechanism of the CyaA toxin from Bordetella pertussis

Post-translational acylation controls the folding and functions of the CyaA RTX toxin.

The Adenylate Cyclase (CyaA) Toxin from Bordetella pertussis Has No Detectable Phospholipase A (PLA) Activity In Vitro.

Department of Structural Biology and Chemistry Department: Paolo de Los Rios, Non-equilibrium protein folding (and unfolding) in the cell.

Translocation and calmodulin-activation of the adenylate cyclase toxin (CyaA) of Bordetella pertussis.

Bioengineering of Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy

Structural disorder and induced folding within two cereal, ABA stress and ripening (ASR) proteins

Membrane-Active Properties of an Amphitropic Peptide from the CyaA Toxin Translocation Region

Synthesis and characterization of tethered lipid assemblies for membrane protein reconstitution (Review)

Exposure to Bordetella pertussis adenylate cyclase toxin affects integrin-mediated adhesion and mechanics in alveolar epithelial cells

Stability, structural and functional properties of a monomeric, calcium-loaded adenylate cyclase toxin, CyaA, from Bordetella pertussis

HDX-MS/NMR DAY

Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis.

From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide

MEMHDX: an interactive tool to expedite the statistical validation and visualization of large HDX-MS datasets.

Molecular Basis of Membrane Association by the Phosphatidylinositol Mannosyltransferase PimA Enzyme from Mycobacteria.

The Tip of the Four N-Terminal α-Helices of Clostridium sordellii Lethal Toxin Contains the Interaction Site with Membrane Phosphatidylserine Facilitating Small GTPases Glucosylation

Biophysical characterization of the translocation process of Bordetella pertussis adenylate cyclase toxin CyaA

Anchoring Sites of Fibrillogenic Peptide Hormone Somatostatin-14 on Plasmonic Nanoparticles.

Molecular crowding

Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion.

Structure and function of RTX toxins.

CyaA secretion, folding and translocation across membrane

Bee venom phospholipase A2 as a membrane-binding vector for cell surface display or internalization of soluble proteins

Development of a new statistical tool for the analysis of large HDX-MS data sets

BiophysiCyaA

The catalytic domains of Clostridium sordellii lethal toxin and related large clostridial glucosylating toxins specifically recognize the negatively charged phospholipids phosphatidylserine and phosphatidic acid

Biochemistry of Macromolecular Interactions

Disorder-to-order transition in the CyaA toxin RTX domain: implications for toxin secretion

Secondary structure reshuffling modulates glycosyltransferase function at the membrane.

Calcium, acylation, and molecular confinement favor folding of Bordetella pertussis adenylate cyclase CyaA toxin into a monomeric and cytotoxic form

Allosteric activation of Bordetella pertussis adenylyl cyclase by calmodulin: molecular dynamics and mutagenesis studies

Bordetella pertussis adenylate cyclase toxin translocation across a tethered lipid bilayer

Characterization of a membrane-active peptide from the Bordetella pertussis CyaA toxin

Molecular crowding stabilizes both the intrinsically disordered calcium-free state and the folded calcium-bound state of a repeat in toxin (RTX) protein

Identification of a region that assists membrane insertion and translocation of the catalytic domain of Bordetella pertussis CyaA toxin

Mean net charge of intrinsically disordered proteins: experimental determination of protein valence by electrophoretic mobility measurements

Estimation of intrinsically disordered protein shape and time-averaged apparent hydration in native conditions by a combination of hydrodynamic methods

Amyloid fibrils formed by the programmed cell death regulator Bcl-xL

Calcium-induced folding of intrinsically disordered repeat-in-toxin (RTX) motifs via changes of protein charges and oligomerization states

Calcium-induced folding and stabilization of the intrinsically disordered RTX domain of the CyaA toxin

Characterization of the regions involved in the calcium-induced folding of the intrinsically disordered RTX motifs from the bordetella pertussis adenylate cyclase toxin

Calmodulin-induced conformational and hydrodynamic changes in the catalytic domain of Bordetella pertussis adenylate cyclase toxin

Clostridium septicum alpha-toxin forms pores and induces rapid cell necrosis

Deciphering membrane insertion of the diphtheria toxin T domain by specular neutron reflectometry and solid-state NMR spectroscopy

Side chain resonances in static oriented proton-decoupled 15N solid-state NMR spectra of membrane proteins

Cellular functions and X-ray structure of anthrolysin O, a cholesterol-dependent cytolysin secreted by Bacillus anthracis

RTX calcium binding motifs are intrinsically disordered in the absence of calcium: implication for protein secretion

Membrane Interaction of botulinum neurotoxin A translocation (T) domain. The belt region is a regulatory loop for membrane interaction

Concerted protonation of key histidines triggers membrane interaction of the diphtheria toxin T domain

Type III secretion effectors of the IpaH family are E3 ubiquitin ligases

Defining the interacting regions between apomyoglobin and lipid membrane by hydrogen/deuterium exchange coupled to mass spectrometry

Behavior of the N-terminal helices of the diphtheria toxin T domain during the successive steps of membrane interaction

Interactions of apomyoglobin with membranes: mechanisms and effects on heme uptake

Structural and functional characterization of an essential RTX subdomain of Bordetella pertussis adenylate cyclase toxin

Conformational states and thermodynamics of alpha-lactalbumin bound to membranes: a case study of the effects of pH, calcium, lipid membrane curvature and charge

Characterization of wild-type recombinant Bet v 1a as a candidate vaccine against birch pollen allergy

Alteration of the tertiary structure of the major bee venom allergen Api m 1 by multiple mutations is concomitant with low IgE reactivity

Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin A is essential for the generation of protective antibodies

Interaction between the two subdomains of the C-terminal part of the botulinum neurotoxin A is essential for the generation of protective antibodies

Anchoring cytokines to tumor cells for the preparation of anticancer vaccines without gene transfection in mice

Clostridium perfringens iota toxin. Mapping of the Ia domain involved in docking with Ib and cellular internalization

Membrane protein insertion regulated by bringing electrostatic and hydrophobic interactions into play. A case study with the translocation domain of diphtheria toxin

Does fusion of domains from unrelated proteins affect their folding pathways and the structural changes involved in their function? A case study with the diphtheria toxin T domain

Prolonged display or rapid internalization of the IgG-binding protein ZZ anchored to the surface of cells using the diphtheria toxin T domain

[Anchoring cytokines to cancer cells using diphtheria toxin: better than immunotherapy by gene transfer?]