Pierre Raia, PhD
One fundamental challenge of cells is to accurately copy their genetic material for cell proliferation. DNA replication is performed by sophisticated multi-protein machines, which must synthesize DNA continuously and copy it accurately at the proper time in the life-cycle of the cell. Many of the replication factors that help replicate DNA act together in a dynamic complex with moving parts referred to as a replisome, loosely analogous to a sewing machine. However, our understanding of how these individual factors interact with one another during the replication processes is still fragmentary. Novel technical developments in Cryo-EM and single-particle analysis combined with protein crystallography and biophysical analyses, now provide biochemists with powerful tools to approach fundamental questions in DNA replication. We aim to approach fundamental questions in DNA replication by using hyperthermophilic Archaea as a model system.
DNA-binding mechanism and evolution of replication protein A
Replication Protein A (RPA) is a heterotrimeric single stranded DNA-binding protein with essential roles in DNA replication, recombination and repair. Little is known about the structure of RPA in Archaea, the third domain of […]
STRUCTURAL BASIS FOR DNA REPLICATION AND REPLICATION-COUPLED DNA REPAIR IN ARCHAEA
One fundamental challenge of cells is to accurately copy their genetic material for cell proliferation. DNA replication is performed by sophisticated multi-protein machines, which must synthesize DNA continuously and copy it accurately at the […]
2023The universal Sua5/TsaC family evolved different mechanisms for the synthesis of a key tRNA modification, Volume 14 .
2023DNA-binding mechanism and evolution of replication protein A., Nat Commun 2023 Apr; 14(1): 2326.
2021Fast and efficient purification of SARS-CoV-2 RNA dependent RNA polymerase complex expressed in Escherichia coli., PLoS One 2021 ; 16(4): e0250610.
2020Novel ribonucleotide discrimination in the RNA polymerase-like two-barrel catalytic core of Family D DNA polymerases., Nucleic Acids Res 2020 12; 48(21): 12204-12218.
2020Structural basis for the increased processivity of D-family DNA polymerases in complex with PCNA., Nat Commun 2020 03; 11(1): 1591.
2019The Extended “Two-Barrel” Polymerases Superfamily: Structure, Function and Evolution., J Mol Biol 2019 09; 431(20): 4167-4183.
2019An updated structural classification of replicative DNA polymerases., Biochem Soc Trans 2019 02; 47(1): 239-249.
2019Structure of the DP1-DP2 PolD complex bound with DNA and its implications for the evolutionary history of DNA and RNA polymerases., PLoS Biol 2019 01; 17(1): e3000122.
2016Shared active site architecture between archaeal PolD and multi-subunit RNA polymerases revealed by X-ray crystallography., Nat Commun 2016 ; 7(): 12227.
+View full list of publications