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© Research
Publication : Rapid communications in mass spectrometry : RCM

Usefulness of an integrated microfluidic device (HPLC-Chip-MS) to enhance confidence in protein identification by proteomics

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Rapid communications in mass spectrometry : RCM - 01 Jan 2006

Hardouin J, Duchateau M, Joubert-Caron R, Caron M

Link to Pubmed [PMID] – 17016832

Rapid Commun. Mass Spectrom. 2006;20(21):3236-44

Nanoflow liquid chromatography/mass spectrometry (nanoLC/MS) has become a current tool in proteomics applications increasingly used in the search for new biomarkers. A new integrated microfluidic device (HPLC-Chip), coupled to ion trap mass spectrometry (ITMS), appears as an innovative and robust tool for improving the identifications commonly performed by nanoLC/MS/MS. We tested this device for the identification of proteins obtained from two-dimensional gel electrophoresis or chromatography. The chip allows the measurement of reproducible retention times that, in association with m/z ratios, was found useful for identifying peptide sequences without ambiguity. A sensitivity increase of a factor of at least 5-fold is obtained compared to the results obtained previously in our laboratory by conventional nanoLC/MS/MS on the same ion trap. We conclude that this recently available microfluidic device can be a valuable tool during biomarker discovery programs, particularly identifying low-abundance proteins.

http://www.ncbi.nlm.nih.gov/pubmed/17016832