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© Emeline Camand
Marquage par immunofluorescence d'astrocytes tumoraux ou astrocytomes (lignée cellulaire humaine U373), montrant en rouge, APC et en vert, la tubuline des microtubules. APC est un supresseur de tumeur qui est impliqué dans la polarisation des astrocytes normaux. La localisation d'APC est altérée dans des lignées de gliomes. Pour essayer de corriger, les dérèglements observés lors de la migration des cellules d'astrocytes tumuraux ou gliomes on cherche à connaitre les mécanismes moléculaires fondamentaux qui controlent la polarisation et la migration cellulaire.
Scientific Fields
Diseases
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Published in 2022 IEEE International Conference on Image Processing (ICIP) - 16 Oct 2022

Yekta Kesenci; Aleix Boquet-Pujadas; Emma van Bodegraven; Sandrine Étienne-Manneville; Elisabeth Labruyère and Jean-Christophe Olivo-Marin

Link to DOI – 10.1109/ICIP46576.2022.9897967

We propose an image based PDE-constrained optimisation framework to compute the dynamical quantities of a cell nucleus undergoing deformation. It allows retrieving the displacement, strain and stress at each pixel of the nuclear domain, as well as the traction force on the boundary. It is based on a mechanical model of the nuclear components and a pair of images documenting the deformation of the cell nucleus. To test our approach, we provide a warping method that produces a second image from an initial one along with the expected mechanical quantities. Both quantitative and qualitative analysis conclude for a significant and consistent improvement of our method over optical flow techniques.

https://ieeexplore.ieee.org/abstract/document/9897967