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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Nucleic acids research

Nucleotide sequence of thrC and of the transcription termination region of the threonine operon in Escherichia coli K12

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Nucleic acids research - 11 Nov 1983

Parsot C, Cossart P, Saint-Girons I, Cohen GN

Link to Pubmed [PMID] – 6316258

Nucleic Acids Res. 1983 Nov;11(21):7331-45

The entire threonine operon (thrABC) of Escherichia coli K12 was cloned, and the nucleotide sequence of the thrC gene and its 3′ flanking region was determined. The translation initiation codon was identified by sequencing the N-terminal part of threonine synthase, the thrC gene product. Analysis of the deduced protein sequence (428 amino acid residues) revealed a region of homology, 35 amino acids long, between the three enzymes encoded by the threonine operon. During examination of the nucleotide sequence of the 1045 base pair fragments following the thrC gene, we detected some potential rho-independent and rho-dependent transcription termination signals.

http://www.ncbi.nlm.nih.gov/pubmed/6316258