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© Research
Publication : Proceedings of the National Academy of Sciences of the United States of America

Monoclonal antibodies to rabbit progesterone receptor: crossreaction with other mammalian progesterone receptors

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Proceedings of the National Academy of Sciences of the United States of America - 01 Nov 1983

Logeat F, Vu Hai MT, Fournier A, Legrain P, Buttin G, Milgrom E

Link to Pubmed [PMID] – 6579531

Proc. Natl. Acad. Sci. U.S.A. 1983 Nov;80(21):6456-9

A mouse was immunized with purified rabbit uterine cytosolic progesterone receptor (specific activity: 3 nmol of steroid bound per mg of protein). After fusion of its spleen cells with Sp2-OAg myeloma cells, supernatants of 11 hybrid cultures were found to react in both an immunoenzymatic test and a double-immunoprecipitation test with the progesterone receptor. Clones were obtained from the five hybrid cells that gave the strongest response in both tests. Antibodies from cell culture supernatants and ascitic fluids were characterized. Three are of the IgG1 and two of the IgG2a isotype. Their apparent affinity for the progesterone receptor was measured by immunoprecipitation in physiological salt conditions. The equilibrium dissociation constants were between 0.1 and 4 nM. All five monoclonal antibodies crossreacted with the rabbit nuclear receptor, the human cytosolic receptor, and other mammalian (rat, guinea pig) but not avian (chicken) cytosolic progesterone receptors. There was no interaction with the glucocorticoid receptor and corticosteroid binding globulin.

https://www.ncbi.nlm.nih.gov/pubmed/6579531