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© Institut Pasteur
Spirochète : bactérie hélicoïdale, flexible et ondulante de longueur variable, non colorable par la coloration de Gram, très mobile (endoflagelles). Trois familles : Spirochaetaceae, Leptospiraceae, et Brachyspiraceae. Principaux genres pathogènes pour l'homme : Borrelia (Borrelia burgdorferi cause de la maladie de Lyme), Treponema (Treponema pallidum cause de la syphillis), Leptospira (Leptospira interrogans serovar icterohaemorrhagiae cause de la maladie de Weil). Image colorisée.
Publication : International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases

Molecular characterization of Leptospira sp by multilocus variable number tandem repeat analysis (MLVA) from clinical samples: a case report

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases - 06 Jul 2015

Pailhoriès H, Buzelé R, Picardeau M, Robert S, Mercier E, Mereghetti L, Lanotte P

Link to Pubmed [PMID] – 26159846

Int. J. Infect. Dis. 2015 Aug;37:119-21

Leptospirosis is a zoonotic infection for which diagnosis is difficult. It has appeared as a global emerging infectious disease over recent years. Genotype determination often requires a Leptospira strain obtained by culture, which is a long and fastidious technique. A method based on multilocus variable number tandem repeat analysis (MLVA) to determine the genotype of Leptospira interrogans, performed directly on blood or urine samples, is proposed. This method was applied to a fatal case of leptospirosis for which the geographical origin of infection was unknown. This technique will allow a genotype to be obtained for L. interrogans, even when cultures remain negative.

http://www.ncbi.nlm.nih.gov/pubmed/26159846