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© Automated cell tracking in a Parhyale hawaiensis embryo. Wolff et al., 2018.
Publication : Current opinion in microbiology

Fluorescence imaging host pathogen interactions: fifteen years benefit of hindsight….

Team: Image Analysis Hub
Team: Photonic BioImaging (UTechS PBI)
Member: Nathalie Aulner
Member: Julien Fernandes
Member: Jean-Yves Tinevez
Member: Audrey Salles
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Current opinion in microbiology - 01 Jun 2018

Aulner N, Danckaert A, Fernandes J, Nicola MA, Roux P, Salles A, Tinevez JY, Shorte SL

Link to Pubmed [PMID] – 29567588

Link to HAL – pasteur-02869379

Link to DOI – 10.1016/j.mib.2018.03.001

Curr Opin Microbiol 2018 Jun; 43(): 193-198

We consider in review current state-of-the-art fluorescence microscopy for investigating the host-pathogen interface. Our perspective is honed from years with literally thousands of microbiologists using the variety of imaging technologies available within our dedicated BSL2/BSL3 optical imaging research service facilities at the Institut Pasteur Paris founded from scratch in 2001. During fifteen years learning from the success and failures of introducing different fluorescence imaging technologies, methods, and technical development strategies we provide here a synopsis review of our experience to date and a synthesis of how we see the future in perspective for fluorescence imaging at the host-pathogen interface.