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© Pierre Lafaye
Astrocytes marqués par des anticorps VHH anti-GFAP. Des anticorps d'alpagas dirigés contre une protéine spécifique des astrocytes, la GFAP (Glial Fibrillary Acidic Protein), ont été obtenus à partir de camélidés immunisés. La partie VHH (partie de l'anticorps qui reconnaît l'antigène) a été exprimée sous forme recombinante chez Escherichia coli.
Publication : Infection and immunity

Antibodies to the leucine-rich repeat region of internalin block entry of Listeria monocytogenes into cells expressing E-cadherin

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Infection and immunity - 01 Dec 1996

Mengaud J, Lecuit M, Lebrun M, Nato F, Mazie JC, Cossart P

Link to Pubmed [PMID] – 8945603

Infect. Immun. 1996 Dec;64(12):5430-3

Internalin, a surface protein essential for entry of Listeria monocytogenes EGD into epithelial cells, was used as an antigen to raise nine monoclonal antibodies. These monoclonal antibodies recognized seven distinct epitopes which were located in three different regions of the protein. Three of them inhibited internalin-mediated entry and recognized the amino-terminal leucine-rich repeat region of the protein, suggesting that this region is essential for entry.

http://www.ncbi.nlm.nih.gov/pubmed/8945603