Présentation
The easy availability of modified ES cells obtained from KOMP and EUCOMM high-throughput gene-targeting mutagenesis programs (mainly JM8.N4, JM8 A3.N1 ES cell lines) has considerably increased the requests for these direct microinjections in the platform. As for the “CIGM internal mutant ES cells”, the external ES mutant cells are microinjected into appropriated mouse blastocysts (mainly BALB/cN) to generate mouse chimeras which will give rise to the desired mutant heterozygous mice.