Thierry Lang joined the Institut Pasteur in Paris in 1983 where he obtained his PhD in 1987 on endocytosis in macrophages in Antoinette Ryter research group. Following a Pasteur-Roux fellowship, Thierry spend two years at the London School of Hygiene and Tropical Medicine (Head of the Unit: Pr. J. Blackwell) as post-doctoral fellow working on ultrastructural analysis of molecular interaction between Leishmania parasites and their vertebrate and insect host and parameters and functions involved in antigen processing and presentation in Leishmania-infected macrophages. He then successively joined Robert Fauve sand Geneviève Milo’s team at Institut Pasteur where he analysed every step of the infectious process that characterizes the niches that Leishmania amastigotes shape at cell or tissue level using the mouse as experimental host. In november 2012, he joined the laboratory of Trypanosomatids Infectious Processes. He was mainly concentrated in the identification and characterization of Leishmania donovani parasitism features at cellular and tissue levels using hamster and mouse experimental models. He had set up in 2011 a translational project involving five partners at the Institut Pasteur and has been coordinating a PTR “Tryp imaging” project (Oct. 2011-March 2014) that put together unique expertise’s to generate efficient tools to monitoring Trypanosomatidae infection in real time at the whole organism level. These approaches may highly improve the sensitivity and the spatial resolution of infectious processes studies and provide reliable means to quantify and visualize parasites in vivo.
Fluorescence Microscopy Workshop III/on cutting-edge technologies
Fluorescence Microscopy Workshop III/on cutting-technologies
Rémi Galland, University of Bordeaux, Interdisciplinary Institute for Neurosciences
Alain Chedotal, Institut de la Vision, Paris
FP7 Targeting the Leishmania kinome for the development of novel anti-parasitic strategies (LeishDrug)
Visceral leishmaniasis is caused by the protozoan parasites Leishmania donovani and Leishmania infantum and is a potentially fatal disease in endemic areas around the world. During the infectious cycle, Leishmania alternate between the insect […]
- 1983: DEA d’Immunologie Approfondie Paris 7
- 1987: Doctorat de Microbiologie l’Université Paris 7 (equivalent to PhD)
- 2005 : Diplôme d’Habilitation à Diriger des Recherches (HDR) Paris 5
- Fellow of the Fondation Roux (1987-1988).
- Assistant (1989-1995) at the Pasteur Institute in the Electron Microscopy Unit (head of the Unit : Pr A. Ryter).
- Posdoctoral Research Fellow (1989-1991) at the London School of Hygiene and Tropical Medicine in the Immunobiology of Parasitic Diseases Unit of the Department of Medical Parasitology (head of the Unit: Pr J. Blackwell).
- Chargé de recherche at the Pasteur Institute:
-1996-1998 in the Cellular Immunophysiology Unit (head of the Unit: Pr R. Fauve).
-1998- oct 2012 in the Immunophysiology and Intracellular Parasitism Unit of the Department of Parasitology (head of the Unit: Dr. G. Milon).
-Since Nov 2012 in the Laboratory of Trypanosomatids Infectious Process (head of the Laboratory: Dr P.Minoprio
Professional activities :
- 2000-2006 Supervision of practical work on the immune response induced by the parasites Leishmania (Pasteur Institute, Paris).
- University Lectures – Pierre et Marie Curie -Paris VI (2005-2010) :
Parasitologie/Mycologie Fondamentale et Médicale : Biologie Moléculaire et cellulaire des agents infectieux et de la cellule hôte.
Interactions Système Immunitaire et Parasites 2010-2013
Major Research Interests 1988-2015
- Endocytosis in macrophages.
- Parasitic processes in mice intradermally inoculated into the ears with L. major or L. amazonensis metacyclic
- Bioluminescent Leishmania major in living C57Bl/6 mice: reliable probes for analysing the concomitant immunity-related processes.
- Long term follow up of Leishmania amazonensis load in C57Bl/6 and DBA/2 mice using bioluminescence imaging in combination with real-time PCR.
- Gene expression profiling of mouse mononuclear phagocytes harbouring Leishmania amazonensis amastigotes. Comparative studies on dendritic leucocytes from either susceptible or resistant mice.
- Lipid metabolism in mouse dendritic leucocytes hosting live Leishmania amazonensis amastigotes.
- Generation and validation of innovative tools for 2D- and 3D-real time imaging of trypanosomatid parasites in their mammalian host.
- Real time imaging of L. donovani in rodents
2013A combined luciferase-expressing Leishmania imaging/RT-qPCR assay provides new insights into the sequential bilateral processes deployed in the ear pinna of C57BL/6 mice, Parasitol. Int. 2014 Feb;63(1):245-53.
2013Reprogramming neutral lipid metabolism in mouse dendritic leucocytes hosting live Leishmania amazonensis amastigotes, PLoS Negl Trop Dis 2013;7(6):e2276.
2013Non-invasive in vivo study of the Trypanosoma vivax infectious process consolidates the brain commitment in late infections, PLoS Negl Trop Dis 2013;7(1):e1976.
2011Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro, PLoS Negl Trop Dis 2011 Sep;5(9):e1323.
2010A combined luciferase imaging and reverse transcription polymerase chain reaction assay for the study of Leishmania amastigote burden and correlated mouse tissue transcript fluctuations, Cell. Microbiol. 2011 Jan;13(1):81-91.
2009Early curative applications of the aminoglycoside WR279396 on an experimental Leishmania major-loaded cutaneous site do not impair the acquisition of immunity, Antimicrob. Agents Chemother. 2010 Mar;54(3):984-90.
2009Sorting of Leishmania-bearing dendritic cells reveals subtle parasite-induced modulation of host-cell gene expression, Microbes Infect. 2010 Jan;12(1):46-54.
2009Imaging Leishmania development in their host cells, Trends Parasitol. 2009 Oct;25(10):464-73.
2008AffyGCQC: a web-based interface to detect outlying genechips with extreme studentized deviate tests, J Bioinform Comput Biol 2008 Apr;6(2):317-34.
1986Vascular irradiation damage: its cellular basis and likely consequences, Br. J. Cancer Suppl. 1986;7:181-91.
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