Sébastien Brier holds an Engineer degree in Biology from Polytech Clermont-Ferrand, and received his Ph.D. in Structural Proteomics at the group of Dr. Eric Forest in 2005 in Grenoble, developing HDX-MS approaches to study the binding of small inhibitors to human mitotic kinesins. He completed postdoctoral work at the Barnett Institute of Chemical Biology in Boston in the group of Professor John. R. Engen, and at the CNRS in Paris, France.
In 2009, he was appointed research investigator at Novartis Vaccines and Diagnostics in Siena, Italy, to create a HDX-MS platform dedicated to the structural analysis of protein vaccines (Epitope mapping) . In Autumn 2012 he came back to Paris as a research associate in Structural Proteomics at Stallergenes-Greer, working on the use of the HDX technology to map IgE epitopes on natural allergens to optimize the design of recombinant vaccines. Currently, Sébastien has implemented and manages the HDX-MS activity at Institut Pasteur in Paris working on membrane-associated proteins involved in infectious disease.
Main Academic Projects and Collaborators
- Structural analysis of B. pertussis CyaA toxin – Collaboration with the group of Alexandre Chenal, Institut Pasteur, Paris.
- Structural analysis of viral IDPs – Collaboration with the group of Sonia Longhi, CNRS, Marseille.
- Epitope mapping – Collaboration with Michel-Robert Popoff , Emmanuel Lemichez, and Alexandre Chenal, Institut Pasteur, Paris.
- Deciphering the structural changes associated with ion binding on the human glutamate transporter – Collaboration with the group of Nicolas Reyes, Institut Pasteur, Paris.
- Identification of protein/aptamer binding sites by HDX-MS – Collaboration with the groupe of Marcel Hollenstein, Institut Pasteur, Paris.
- Identification of antigen binding sites on natural allergens. Collaboration with the pharmaceutical company Stallergenes-Greer, Antony, France.