Tuberculosis still remains a major threat for public health, with one-third of the world population latently infected, and more than one million deaths per year according to the WHO. Among the factors that have […]
Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved ways to their synthesis include the oxidative decarboxylation of α-ketoacids carried out by three distinct enzymatic machineries: the pyruvate dehydrogenase […]
Type II protein secretion mechanism
Many Gram-negative bacteria secrete stably folded or even multimeric proteins across the outer membrane. The secreted substrates are often virulence factors like hydolases or toxins that contribute to the ability of bacteria to adapt […]
Structural studies of type II topoisomerases
Our main research projects focus on structural and functional studies of M. tuberculosis DNA gyrase and aim to understand the structural dynamics of DNA recognition by DNA gyrase and the quinolone resistance mechanisms […]
PknA/PknB in mycobacterial cell division
The genes pknA and pknB are located near the origin of replication in M. tuberculosis and are part of a conserved operon that also includes pstP (coding the only known Ser/Thr protein phosphatase […]
Control of glutamate metabolism in mycobacteria
We recently demonstrated the existence of a phosphorylation-dependent control of glutamate metabolism in mycobacteria, in collaboration with H. O’Hare’s group in Leicester (O’Hare et al., 2008). Our results were supported by similar observations […]
2017Structural Coupling between Autokinase and Phosphotransferase Reactions in a Bacterial Histidine Kinase, Structure 2017 Jun;25(6):939-944.e3.
2017PknG senses amino acid availability to control metabolism and virulence of Mycobacterium tuberculosis, PLoS Pathog. 2017 May;13(5):e1006399.
2017The crystal structure of PknI from Mycobacterium tuberculosis shows an inactive, pseudokinase-like conformation, FEBS J. 2017 02;284(4):602-614.
2016Modification in hydrophobic packing of HAMP domain induces a destabilization of the auto-phosphorylation site in the histidine kinase CpxA, Biopolymers 2016 Oct;105(10):670-82.
2016Bidirectional Allosteric Communication between the ATP-Binding Site and the Regulatory PIF Pocket in PDK1 Protein Kinase, Cell Chem Biol 2016 Sep;.
2016Ser/Thr phosphorylation regulates the Fatty Acyl-AMP Ligase activity of FadD32, an essential enzyme in mycolic acid biosynthesis, J. Biol. Chem. 2016 Sep;.
2016Molecular Basis of Membrane Association by the Phosphatidylinositol Mannosyltransferase PimA Enzyme from Mycobacteria, J. Biol. Chem. 2016 Jul;291(27):13955-63.
2015Structural Basis of Pullulanase Membrane Binding and Secretion Revealed by X-Ray Crystallography, Molecular Dynamics and Biochemical Analysis, Structure 2016 Jan;24(1):92-104.
2015Thiophenecarboxamide Derivatives Activated by EthA Kill Mycobacterium tuberculosis by Inhibiting the CTP Synthetase PyrG, Chem. Biol. 2015 Jul;22(7):917-27.
2015Molecular Basis of the Activity and the Regulation of the Eukaryotic-like S/T Protein Kinase PknG from Mycobacterium tuberculosis, Structure 2015 Jun;23(6):1039-48.
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