We are interested in investigating the effects of molecular crowding, i.e., excluded volume effects, mimicking the effects of high concentrations of macromolecular solutes found in biological fluids on biochemical properties of proteins. In particular, […]
CyaA secretion, folding and translocation across membrane
CyaA, a 1706 residue-long protein, is one of the major virulence factors produced by B. pertussis and plays an important role in the early stages of respiratory tract colonization. This toxin uses an original […]
Regulatory roles of signaling Nucleotides
Other research projects are aimed at exploring the regulatory role of different nucleotides such ppGpp and pppGpp that are both thought to act as second messengers in many complex cellular responses to nutritional […]
2018Calcium-dependent disorder-to-order transitions are central to the secretion and folding of the CyaA toxin of Bordetella pertussis, the causative agent of whooping cough, Toxicon 2018 Jul;149:37-44.
2016Actin activates Pseudomonas aeruginosa ExoY nucleotidyl cyclase toxin and ExoY-like effector domains from MARTX toxins, Nat Commun 2016 Dec;7:13582.
2015Cryptococcosis Serotypes Impact Outcome and Provide Evidence of Cryptococcus neoformans Speciation, MBio 2015;6(3):e00311.