The project will be conducted in a highly stimulating, interdisciplinary and international environment in the department of Bacteriology of the Institut Pasteur, Paris. The successful candidate will start in 2018 the study of the interplay of bacterial toxins with the cellular ubiquitin and proteasome systems and consequences in infection. Funding for an additional 1 year will be dependent on the CV of the applicant and advancement of the project.
Post-translational modifications of proteins by ubiquitination are central reactions in the maintenance of cellular homeostasis, quality control of the proteome and innate immune signaling in reaction to the attacks by pathogens, which frequently have acquired the ability to divert ubiquitin-mediated reactions to their advantage. It is therefore essential to define the interplay between bacterial virulence factors and ubiquitin-mediated cellular regulations. The study of the cytotoxic necrotizing factor-1 (CNF1) from pathogenic Escherichia coli, a toxin that catalyzes the activation of Rho GTPases, has been instrumental in deciphering the regulation of Rho GTPases by the ubiquitin-proteasome systems. The regulation of Rho GTPases by ubiquitination has emerged these recent years as a critical determinant in their regulatory function on the actin cytoskeleton, on cell growth and fate, and for the control of inflammatory signaling pathways. However, the overall impact of Rho-activating toxins on the dynamics of proteome ubiquitination and its involvement in cell-autonomous anti-microbial functions is less appreciated. This will be studied using cutting edge proteomic and imaging techniques.
We are looking for a highly motivated candidate with good track record, independent thinking and competence in data organization & manuscript preparation. Experience in proteomics and skills in basic molecular biology and protein biochemistry are required, along with interest in cell biology. Previous experience in microbiology would be appreciated but is not mandatory.
How to apply : please send a full CV, brief statement of previous research accomplishments (max 1 page) and 2 reference letters : firstname.lastname@example.org
Closing date to submit applications: 15th September 2017
Selected candidates will be contacted: 15th October 2017
Employment start date: 1st January 2018
Contract length: 2 (+ 1) years
Institution: Institut Pasteur
Laboratory of Bacterial Toxins
25 rue du Dr Roux