The Biomaterials & Microfluidics core facility of Institut Pasteur is opening a position for developing a new Organ-on-Chip device capable of recapitulating the alveolar microenvironment. The objective is to develop a new tool that could help better understanding SARS-CoV-2 infection spreading along the respiratory tract and the induced immune responses. Practically, the selected candidate will work at the interface of cell biology, microfluidics and organoid technologies in order to deliver an alveolar organotypic model susceptible to SARS-CoV-2 challenges. Application with other infectious diseases could be sought in a later time.
The project entails developing an in vitro organ-on-chip model capable of recapitulating the hallmarks of SARS-CoV-2 infection in different parts of the lung (primarily the alveoli). The first step will consist in the development of the model. There, the selected candidate will have to harvest primary lung cells, amplify them in organoid culture, and steer them towards alveolar differentiation. After thorough characterization (Immunochemistry, FACS, scRNAseq….), the second step will consist in loading the produced cells onto a microfluidic device, to assess lung cell functions and then analyze the response to SARS-CoV-2 infection.
Skills and Expertise
The proposed project is clearly multidisciplinary. It will entail working at the interface of multiple fields of investigation including organoids, lung physiology and development, microfluidics and immunity. Expertise in the field of lung organotypic models or lung development is expected. Advanced understanding (theoretical and practical) of primary cell culture techniques is required. Good understanding of organoids, 3D cell culture and microfluidics would be a strong asset.
The candidate must be highly motivated, creative and capable of independent thinking. He/she should be able to collaborate efficiently with scientists across multiple labs specialized in virology, cell biology and drug screening. The ability to work independently and good communication skills in spoken and written English will also be important.
The candidate should have a PhD degree in cell biology or bioengineering with a proven record in the fields of lung physiology and/or development.
The project will be based on a collaboration between different labs and core facilities at Institut Pasteur. The successful candidate will be hosted by the Biomaterials & Microfluidics core facility and will work very closely with other labs on the campus including virologists, cell biologists and chemists.
Applicants should send their CV, a summary of previous research experience and the names of two references to Samy Gobaa (email@example.com)
Funding body: Institut Pasteur. Duration: 12 months