Timeline

Olivera Francetic

Group Leader

08 Dec 2023

publication

Membrane platform protein PulF of the Klebsiella type II secretion system forms a trimeric ion channel essential for endopilus assembly and protein secretion.

02 May 2023

publication

The periplasmic coiled coil formed by the assembly platform proteins PulL and PulM is critical for function of the Klebsiella type II secretion system.

13 Apr 2023

project

Electrogenetic control of bacterial metabolism, communication, and biofilm formation

13 Apr 2023

project

Bacterial nanowires

27 Mar 2023

publication

Bacterial Type II Secretion System and Its Mitochondrial Counterpart.

02 Feb 2023

publication

Structure and dynamic association of an assembly platform subcomplex of the bacterial type II secretion system.

01 Feb 2023

news

Bacterial pathogenicity: improving understanding of an essential process – molecule secretion

01 Oct 2021

publication

1 H, 15 N and 13 C resonance assignments of the C-terminal domain of PulL, a component of the Klebsiella oxytoca type II secretion system

11 Sep 2021

publication

Computational and biochemical analysis of type IV pilus dynamics and stability.

19 Aug 2021

publication

1H, 15 N and 13C resonance assignments of the C-terminal domain of PulL, a component of the Klebsiella oxytoca type II secretion system.

19 May 2021

publication

Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system.

29 Nov 2019

publication

Structure and function of minor pilins of type IV pili.

31 Jul 2019

project

Biogenesis of extracytoplasmic proteins in Gram-negative bacteria

31 Jul 2019

project

Synergy_T2SS

02 May 2019

publication

Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.

09 Apr 2019

publication

Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.

21 Jan 2019

publication

Functional reconstitution of the type IVa pilus assembly system from enterohaemorrhagic Escherichia coli.

01 Nov 2018

publication

Tagging the type VI secretion system.

07 Jun 2018

event

Enterobacterial type IV pili: structure, assembly and molecular function

23 May 2018

publication

Protein polarization driven by nucleoid exclusion of DnaK(HSP70)-substrate complexes.

18 Jan 2018

publication

Functional role of the type 1 pilus rod structure in mediating host-pathogen interactions.

01 Jan 2018

publication

Analysis of Bacterial Pilus Assembly by Shearing and Immunofluorescence Microscopy.

09 Oct 2017

publication

Structure of the calcium-dependent type 2 secretion pseudopilus.

01 Oct 2017

publication

1H, 15N and 13C resonance assignments and secondary structure of PulG, the major pseudopilin from Klebsiella oxytoca type 2 secretion system.

01 Jul 2017

publication

The trans-envelope architecture and function of the type 2 secretion system: new insights raising new questions.

31 May 2017

publication

Tracking Proteins Secreted by Bacteria: What’s in the Toolbox?

21 May 2017

publication

Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD.

21 May 2017

publication

Polar N-terminal Residues Conserved in Type 2 Secretion Pseudopilins Determine Subunit Targeting and Membrane Extraction Steps during Fibre Assembly

17 Apr 2017

publication

Polar N-terminal Residues Conserved in Type 2 Secretion Pseudopilins Determine Subunit Targeting and Membrane Extraction Steps during Fibre Assembly.

06 Jan 2017

publication

Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD.

05 Jul 2016

publication

Pseudopilin residue E5 is essential for recruitment by the type 2 secretion system assembly platform.

06 Jan 2016

news

The pullulanase secretion signal

10 Dec 2015

publication

Structural Basis of Pullulanase Membrane Binding and Secretion Revealed by X-Ray Crystallography, Molecular Dynamics and Biochemical Analysis

04 Sep 2015

project

Type II protein secretion mechanism

22 Jul 2015

project

FiberSpace

11 May 2015

team

Macromolecular Systems

11 May 2015

team

Biochemistry of Macromolecular Interactions

27 Mar 2014

publication

Distinct docking and stabilization steps of the Pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibers

03 Jan 2014

publication

Type II secretion system: a magic beanstalk or a protein escalator

27 Mar 2013

publication

The type II secretion system – a dynamic fiber assembly nanomachine

15 Dec 2012

publication

¹H, ¹⁵N and ¹³C resonance assignments of PpdD, a type IV pilin from enterohemorrhagic Escherichia coli

25 Sep 2012

publication

Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation

09 Dec 2011

publication

Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation

27 Nov 2010

publication

Modeling pilus structures from sparse data

02 Jul 2010

publication

Detailed structural and assembly model of the type II secretion pilus from sparse data

17 Apr 2009

publication

The haloprotease CPI produced by the moderately halophilic bacterium Pseudoalteromonas ruthenica is secreted by the type II secretion pathway

28 Jul 2008

publication

Spx mediates oxidative stress regulation of the methionine sulfoxide reductases operon in Bacillus subtilis

10 Jan 2008

publication

Cytoplasmic targeting of IpaC to the bacterial pole directs polar type III secretion in Shigella

01 Nov 2007

publication

Intestinal adherence associated with type IV pili of enterohemorrhagic Escherichia coli O157:H7

01 Apr 2006

publication

Green Fluorescent Chimeras Indicate Nonpolar Localization of Pullulanase Secreton Components PulL and PulM

01 Oct 2005

publication

Towards the identification of type II secretion signals in a nonacylated variant of pullulanase from Klebsiella oxytoca

01 Apr 2004

publication

Getting out: protein traffic in prokaryotes

15 Dec 2000

publication

Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion

01 Mar 2000

publication

The ChiA (YheB) protein of Escherichia coli K-12 is an endochitinase whose gene is negatively controlled by the nucleoid-structuring protein H-NS

15 Dec 1999

publication

DNA region responsible for transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene by CRP and PAA

01 Apr 1998

publication

Protein secretion in Escherichia coli K-12: dead or alive?

01 Feb 1998

publication

A second prepilin peptidase gene in Escherichia coli K-12

11 Jun 1997

publication

Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria–a review

01 Jan 1997

publication

Pullulanase: model protein substrate for the general secretory pathway of gram-negative bacteria

01 Oct 1996

publication

Escherichia coli SecB stimulates export without maintaining export competence of ribose-binding protein signal sequence mutants

01 Jun 1996

publication

The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins

01 Dec 1993

publication

On the puzzling arrangement of the asymmetric MalT-binding sites in the MalT-dependent promoters

01 Jul 1993

publication

prlA suppression of defective export of maltose-binding protein in secB mutants of Escherichia coli

01 Apr 1993

publication

A mutation of Escherichia coli SecA protein that partially compensates for the absence of SecB

01 Apr 1993

publication

A little guide for advanced search:

Membrane platform protein PulF of the Klebsiella type II secretion system forms a trimeric ion channel essential for endopilus assembly and protein secretion.

The periplasmic coiled coil formed by the assembly platform proteins PulL and PulM is critical for function of the Klebsiella type II secretion system.

Electrogenetic control of bacterial metabolism, communication, and biofilm formation

Bacterial nanowires

Bacterial Type II Secretion System and Its Mitochondrial Counterpart.

Structure and dynamic association of an assembly platform subcomplex of the bacterial type II secretion system.

Bacterial pathogenicity: improving understanding of an essential process – molecule secretion

1 H, 15 N and 13 C resonance assignments of the C-terminal domain of PulL, a component of the Klebsiella oxytoca type II secretion system

Computational and biochemical analysis of type IV pilus dynamics and stability.

1H, 15 N and 13C resonance assignments of the C-terminal domain of PulL, a component of the Klebsiella oxytoca type II secretion system.

Analysis of diverse eukaryotes suggests the existence of an ancestral mitochondrial apparatus derived from the bacterial type II secretion system.

Structure and function of minor pilins of type IV pili.

Biogenesis of extracytoplasmic proteins in Gram-negative bacteria

Synergy_T2SS

Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.

Structure and Assembly of the Enterohemorrhagic Escherichia coli Type 4 Pilus.

Functional reconstitution of the type IVa pilus assembly system from enterohaemorrhagic Escherichia coli.

Tagging the type VI secretion system.

Enterobacterial type IV pili: structure, assembly and molecular function

Protein polarization driven by nucleoid exclusion of DnaK(HSP70)-substrate complexes.

Functional role of the type 1 pilus rod structure in mediating host-pathogen interactions.

Analysis of Bacterial Pilus Assembly by Shearing and Immunofluorescence Microscopy.

Structure of the calcium-dependent type 2 secretion pseudopilus.

1H, 15N and 13C resonance assignments and secondary structure of PulG, the major pseudopilin from Klebsiella oxytoca type 2 secretion system.

The trans-envelope architecture and function of the type 2 secretion system: new insights raising new questions.

Tracking Proteins Secreted by Bacteria: What’s in the Toolbox?

Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD.

Polar N-terminal Residues Conserved in Type 2 Secretion Pseudopilins Determine Subunit Targeting and Membrane Extraction Steps during Fibre Assembly

Polar N-terminal Residues Conserved in Type 2 Secretion Pseudopilins Determine Subunit Targeting and Membrane Extraction Steps during Fibre Assembly.

Prepore Stability Controls Productive Folding of the BAM-independent Multimeric Outer Membrane Secretin PulD.

Pseudopilin residue E5 is essential for recruitment by the type 2 secretion system assembly platform.

The pullulanase secretion signal

Structural Basis of Pullulanase Membrane Binding and Secretion Revealed by X-Ray Crystallography, Molecular Dynamics and Biochemical Analysis

Type II protein secretion mechanism

FiberSpace

Macromolecular Systems

Biochemistry of Macromolecular Interactions

Distinct docking and stabilization steps of the Pseudopilus conformational transition path suggest rotational assembly of type IV pilus-like fibers

Type II secretion system: a magic beanstalk or a protein escalator

The type II secretion system – a dynamic fiber assembly nanomachine

¹H, ¹⁵N and ¹³C resonance assignments of PpdD, a type IV pilin from enterohemorrhagic Escherichia coli

Heterologous assembly of type IV pili by a type II secretion system reveals the role of minor pilins in assembly initiation

Minor pseudopilin self-assembly primes type II secretion pseudopilus elongation

Modeling pilus structures from sparse data

Detailed structural and assembly model of the type II secretion pilus from sparse data

The haloprotease CPI produced by the moderately halophilic bacterium Pseudoalteromonas ruthenica is secreted by the type II secretion pathway

Spx mediates oxidative stress regulation of the methionine sulfoxide reductases operon in Bacillus subtilis

Cytoplasmic targeting of IpaC to the bacterial pole directs polar type III secretion in Shigella

Intestinal adherence associated with type IV pili of enterohemorrhagic Escherichia coli O157:H7

Green Fluorescent Chimeras Indicate Nonpolar Localization of Pullulanase Secreton Components PulL and PulM

Towards the identification of type II secretion signals in a nonacylated variant of pullulanase from Klebsiella oxytoca

Getting out: protein traffic in prokaryotes

Expression of the endogenous type II secretion pathway in Escherichia coli leads to chitinase secretion

The ChiA (YheB) protein of Escherichia coli K-12 is an endochitinase whose gene is negatively controlled by the nucleoid-structuring protein H-NS

DNA region responsible for transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene by CRP and PAA

Protein secretion in Escherichia coli K-12: dead or alive?

A second prepilin peptidase gene in Escherichia coli K-12

Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria–a review

Pullulanase: model protein substrate for the general secretory pathway of gram-negative bacteria

Escherichia coli SecB stimulates export without maintaining export competence of ribose-binding protein signal sequence mutants

The cryptic general secretory pathway (gsp) operon of Escherichia coli K-12 encodes functional proteins

On the puzzling arrangement of the asymmetric MalT-binding sites in the MalT-dependent promoters

prlA suppression of defective export of maltose-binding protein in secB mutants of Escherichia coli

A mutation of Escherichia coli SecA protein that partially compensates for the absence of SecB

Highly selective binding of nascent polypeptides by an Escherichia coli chaperone protein in vivo