Zebrafish Projects Hub

Présentation

The Zebrafish Projects Hub (Zorgl’hub) aims to provide Pasteur research groups with the possibility to conduct short term zebrafish trial projects,

as a functional complement to existing projects using other models/cells or
to obtain proof of principle evidence to support grant applications.

Why the zebrafish ?

The zebrafish (Danio rerio) is an ideal vertebrate model to study developmental, physiological and pathological processes, including infectious diseases. It is genetically close to human, and shares most molecular and cellular pathways with mammals, including immune cells and pathways. It is excellent for live imaging, high throughput screening studies, gene knockdown or overexpression in embryos. This offers the unique opportunity to study in vivo the molecular and cellular underpinnings of physiological processes.

Zebrafish larva (72 hours, approx. size 3 mm) (left) and adult (3 months, approx. size 3 cm) (right).

Who ?

Unit Zebrafish Neurogenetics (ZEN): developmental biology / cell biology / neuroscience
Resp. : Laure Bally-Cuif

What services?

Production of zebrafish embryos, juveniles and adults (live animals and tissues)
Zebrafish husbandry
Support to developmental biology, cell biology and neuroscience projects

Prices cover only consumables and equipment depreciation.

Example of a pipeline:

a-d: Experimental pipeline: breeding (a), microinjections (b,c) and embryo analysis (d). e: her5:mCherry;cmcl2:gfp transgenic embryo used for the analysis of neural phenotypes (her5: midbrain-hindbrain boundary of the neural tube, cmcl2: cardiac myosin, used as a transgenic reporter). f,g: examples of larvae analysed for pathogen infections: larva infected with red bacteria and showing green neutrophils (f) and larvae showing red macrophages and green neutrophils (g).

Current projects

Identification of Tunneling Nanotubes in neural stem cells in vivo Chiara Zurzolo, Shiyu Liu, Department of Cell Biology & Infection, Institut Pasteur

Development of a model of oral Listeria infection in zebrafish Enzo Gadin, Olivier Disson, Marc Lecuit, Biology of Infection Unit, Institut Pasteur

Previous projects

Identification of tunneling nanotubes in zebrafish embryo, Chiara Zurzolo, Olga Korenkova, Department of Cell Biology & Infection, Institut Pasteur, 2018-2022

Investigation of principles of microbiota-based colonization resistance in zebrafish larvae, Jean-Marc Ghigo, David Perez-Pascual, Rebecca Stevick, Department of Microbiology, Institut Pasteur, 2018-2021

Zebrafish larvae immune response after Yersinia ruckeri infection, Jean-Marc Ghigo, David Perez-Pascual, Rebecca Stevick, Department of Microbiology, Institut Pasteur, 2020-2023

Related publications

Olga Korenkova, Shiyu Liu, Inès Prlesi, Anna Pepe, Shahad Albadri, et al.. Tunneling nanotubes
enable intercellular transfer in zebrafish embryos. 2024. pasteur-04626630

Adade EE, Stevick RJ, Pérez-Pascual D, Ghigo JM, Valm AM. Gnotobiotic zebrafish microbiota display inter-individual variability affecting host physiology. bioRxiv [Preprint]. 2023 Feb 1:2023.02.01.526612. doi: 10.1101/2023.02.01.526612. PMID: 36778358; PMCID: PMC9915576.

Stevick, R.J., Audrain, B., Bedu, S. et al. Anti-diarrheal drug loperamide induces dysbiosis in zebrafish microbiota via bacterial inhibition. Microbiome 11, 252 (2023). https://doi.org/10.1186/s40168-023-01690-z

Protocole d'accès

To place a request, please contact zorglhub@pasteur.fr.
We will plan a scientific discussion with you and will then provide you with timeline and quote.
Costs are elligible for any funding source, including EC contracts.

Criteria for selection include:

technical feasibility and scientific soundness for using the zebrafish model for the project
fitting with the scientific expertise of the Zorgl’hub teams (alternatively, more appropriate labs can be suggested)
project feasibility with available equipment on site (in the fish facility and Zorgl’hub team, or at Institut Pasteur)

The Zebrafish Projects Hub team should be acknowledged in publications but no co-authorship is requested (unless the project develops into a collaboration).

Un petit guide pour l'utilisation de la recherche avancée :