Lien DOI – 10.1109/ISBI.2016.7493429
2016 IEEE 13th International Symposium on Biomedical Imaging (ISBI), Prague, 2016, pp. 980-983
Measuring the biophysical properties of living cells is essential to understand their underlying cytoskeletal machinery. Here we describe a robust extension of our previous algorithm for the intra-cellular measurement of pressure, velocity and forces inside living cells. We first consider a compressible fluid model to cope with out-of-plane intracellular flow in the 2D case, and also propose a 3D version of the algorithm for multi-plane imaging. Results demonstrate the high potential of the approach, giving access to biophysical measures that cannot be achieved using other experimental techniques.