Microbiology Course Institut Pasteur
I have been appointed in 2010 then Co-director together with Dr Françoise Norel-Bozouklian in 2014 of this Microbiology course. This nine-week theorical and practical course presents the latest advances in molecular and cellular microbiology. […]
Institut Pasteur Microbiology Course
Françoise Norel has been appointed Co-director of the Institut Pasteur Microbiology Course in 2014. This course is held annually and is part of master trainings proposed by 3 universities: Pierre and Marie Curie (Paris […]
Small membrane proteins in quiescent Salmonella cells
One novel project of our laboratory is to characterize the function of small proteins specifically expressed in nongrowing Salmonella cells. Many of these proteins have a wide phylogenetic distribution in bacteria and eukaryotes and […]
Downregulation of metabolic and membrane functions in quiescent cells
In Salmonella enterica serovar Typhimurium and many other Gram negative bacteria, SigmaS (RpoS) activates global gene expression and controls adaptive functions including stress resistance, biofilm formation and virulence. SigmaS also downregulates housekeeping metabolic and […]
In solution conformation of the SigmaS subunit of RNA polymerase
There is no tridimentional structure available for free sigma factors. We have recently probed the conformation of SigmaS in solution by analytical ultracentrifugation, small angle X-ray scattering and Hydrogen-Deuterium exchange coupled with mass spectrometry.
Characterization of the SigmaS network by RNA sequencing and identification of SigmaS-controlled small regulatory RNAs in Salmonella
We have used directional RNA-sequencing and complementary assays to explore the SigmaS-dependent transcriptome of Salmonella enterica serovar Typhimurium. This study confirms the large regulatory scope of SigmaS and provides insights into its physiological functions […]
Crl: a “binding and deliver” SigmaS chaperone
Regulation of many key biological processes in bacteria involves the interplay between Sigma factors and anti-Sigma factors that typically attenuate Sigma factor function by restricting its access to RNA polymerase (RNAP). In contrast, our […]
2021Escherichia coli Genomic Diversity within Extraintestinal Acute Infections Argues for Adaptive Evolution at Play., mSphere 2021 Jan; 6(1): .
2018The stress sigma factor of RNA polymerase RpoS/σ is a solvent-exposed open molecule in solution, Biochem. J. 2018 01;475(1):341-354.
2017Proteome remodelling by the stress sigma factor RpoS/σS in Salmonella: identification of small proteins and evidence for post-transcriptional regulation., Sci Rep 2017 05; 7(1): 2127.
2016Recent advances in the characterization of Crl, the unconventional activator of the stress sigma factor σS/RpoS, Biomol Concepts 2016 May;.
2015Binding interface between the Salmonella σ(S)/RpoS subunit of RNA polymerase and Crl: hints from bacterial species lacking crl, Sci Rep 2015;5:13564.
2015(1)H, (13)C and (15)N resonance assignments of σ(S) activating protein Crl from Salmonella enterica serovar Typhimurium, Biomol NMR Assign 2015 May;.
2015Characterization of the RpoS status of clinical isolates of Salmonella enterica, Appl. Environ. Microbiol. 2003 Aug;69(8):4352-8.
2015Crl activates transcription initiation of RpoS-regulated genes involved in the multicellular behavior of Salmonella enterica serovar Typhimurium, J. Bacteriol. 2006 Jun;188(11):3983-94.
2015Repressor activity of the RpoS/σS-dependent RNA polymerase requires DNA binding, Nucleic Acids Res. 2015 Feb;43(3):1456-68.
2014Structural and functional features of Crl proteins and identification of conserved surface residues required for interaction with the RpoS/σS subunit of RNA polymerase, Biochem. J. 2014 Oct;463(2):215-24.
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