Mutualized Platform for Microbiology (P2M) is open to all the reference laboratories of the Institut Pasteur, in Paris and within the International Network (IPIN) and provide easy access to next generations multi-pathogenic sequencing (Illumina and Ion Torrent technologies).
The sequencing is made according to a unique library manufacturing protocol whatever the pathogen (bacteria, viruses, fungi or parasites). This allows optimization and shortening of the process and, thus place this technology in the panel of microbiological public health surveillance tools. Bio-IT specialists commissioned by the C3BI performed the quality controls of the sequences and can also help laboratories, if needed, to implement routine sequence analyses.
An automatic extractor is also available. A fast and unique protocol can be used not only to prepare DNA dedicated to NGS, but also for other purposes such as nucleic acid extraction from big series within the framework of epidemics. P2M has just acquired a new robot which will quickly increase its sequence production capacities. Other small equipment complete the whole arsenal and can be used in “self-service store”. A MALDI-TOF mass spectrometry is also available.
The platform follows the Insurance Quality standards of the Institut Pasteur and joins in the scope of activities of the LREMS (Laboratory of Reference and Expertise Multi-site, a structure including, within a single entity, all the National Reference Centers and WHO-Collaborative Centers present on campus, and the Urgent Response to Biological Threats (CIBU)).