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About

At the Imaging Facility of the Hearing Institute, we offer diverse systems to meet your imaging needs. The available instruments include:

  • Epifluorescence microscopes
  • Confocal microscopes
  • Spinning disk microscopes
  • STED microscopes
  • Two-photon microscopes
  • Benchtop electron microscope

These instruments allow you to observe samples at various levels, ranging from entire organoids to the fine details of intracellular structures (~50 nm lateral resolution in optical microscopy).

We provide support and services in the image acquisition process for both fixed and live samples. We also assist with image treatment, analysis, and interpretation.

We strive to offer guidance in sample preparation and manage the cell culture and histology rooms. Our goal is to establish protocols for sample preparation and labeling tailored to the typical research activities of the Hearing Institute, also work with the institute’s teams to develop home-made optical systems tailored for live imaging. We offer these resources to both the facility and individual research teams.

Equipments

Two photon miniscope • LABMaker •

Miniature two-photon microscope allowing the observation of neuronal activity in freely moving mice (fixed laser at 920 nm).

Confocal Stellaris 5 Leica • Leica • Stellaris 5

Confocal microscope compatible with fixed and live cells. Gentle live cell imaging through efficient signal acquisition at the lowest required levels of illumination.  10x, NA 0.3, WD: 11mm, FLUOTAR 20x, NA 0.75, WD: 0.62mm, […]

Two photon microscope • Home-Made •

Completely home-made flexible two photon microscope with rotating head, two collection colors (green and red) and tunable excitation laser (680-1300 nm). 

Epi-fluorescence Microscope • Zeiss • Axiovert

Epi-fluorescence microscope Axiovert Excitation: 375nm/490nm/550nm/633nm Cubes : DAPI/GFP/RFP/Cy5 CMOS Camera Objectives : 10x/03 PH1 WD :5.1mm (Contraste de phase) 20x/0.75 40x/0.75 63x/1.4 Oil

STED microscope • Aberrior Instruments • Stedycon

Confocal scanning microscope with 4 laser lines equiped with a STED module that allows up to 30 nm lateral spatial resolution at two colors (orange and red). It is really simple to use but […]

Spinning-disk Microscope • Gataca • W1

Scanning microscope that allows the acquisition of images with optical sectioning with an extended field of view at very high speed and low photo-toxicity. Equipped with a super-resolution module it allows acquiring images with […]

Confocal Nikon A1HD25 • Nikon • A1HD25

Scanning microscope that allows the acquisition of images with optical sectioning and an extended field of view. Equipped with resonant scanners it is a high speed and low photo-toxicity microscope. It is the ideal […]

Confocal Zeiss LSM 700 • Zeiss • LSM 700

Scanning microscope that allows the acquisition of images with optical sectioning. Suitable for fixed samples showing low expression. Excitation: 405nm/488nm/561nm/633nm Objectives : 5x N0.16 10x NA0.3 20x NA 0.8 25x 0.8W 63x NA1.4 Oil

Confocal Zeiss LSM 900 Airyscan • Zeiss • LSM 900 Airyscan

Scanning microscope that allows the acquisition of images with optical sectioning. Compatible with fixed and living samples with low levels of expression it uses low excitation intensities to gently imaging your samples in 3D […]

Compact electronic microscope (SEM) • Hitachi • SU1510

System used for the observation of intracellular structures in fixed tissue. Detector SE : 0nm à 30kV (High Vacuum Mode) – 10nm à 3kV (High Vacuum Mode) Detector BSE : 0nm à 30kV (Low Vacuum Mode) […]

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