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This type of transgenesis consists in the microinjection of the gene of interest into the pronucleus of fertilized eggs to generate transgenic mice expressing the transgene. The microinjected transgenes, which integrated randomly into the […]

The nucleases (ZFN pairs, TALE pairs, Cas9/CRISPR system) are microinjected, mainly in its mRNA form, into the pronucleus and/or the cytoplasm of one-cell mouse/rat embryos in order to get the desired targeted modification. These […]

The microinjection of the CRISPR/Cas9 system in one-cell embryos is actually revolutionizing the in vivo genome-engineering field because of its big efficiency for KO/ KI (when a donor DNA matrix co-injected with the CRISPR/CAS9 […]

Lentiviral transgenesis is a special type of classical/additive transgenesis. The transgene, inserted in a lentiviral construct, is microinjected directly under the zona pellucida of one-cell embryos. Then viruses are able to enter directly the […]

The easy availability of modified ES cells obtained from KOMP and EUCOMM high-throughput gene-targeting mutagenesis programs (mainly JM8.N4, JM8 A3.N1 ES cell lines) has considerably increased the requests for these direct microinjections in the […]

We have an increasing experience in modification on rat genome using lentiviral vectors and Zinc Finger Nucleases (ZFNs). Rat models are more convenient for surgery, due to their medium size, and constitute a robust […]

A gene targeting vector is transfected into the desired mouse ES cells to modify an endogenous gene by homologous recombination. The mutant ES cells thus obtained are subsequently microinjected into mouse blastocysts for the […]

Malaria is a tropical disease due to a parasite named Plasmodium. It is a global health problem, still causing more than 450.000 deaths each year (mostly African children) and over 200 million infections (http://www.who.int/malaria/en/). […]