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© Therese Couderc, Marc Lecuit
Publication : Expert review of molecular diagnostics

The potential of whole genome NGS for infectious disease diagnosis

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Expert review of molecular diagnostics - 07 Nov 2015

Lecuit M, Eloit M

Link to Pubmed [PMID] – 26548640

Expert Rev. Mol. Diagn. 2015;15(12):1517-9

Non-targeted identification of microbes is now possible directly in biological samples, based on whole-genome-NGS (WG-NGS) techniques that allow deep sequencing of nucleic acids, data mining and sorting out of sequences of pathogens without any a priori hypothesis. WG-NGS was first only used as a research tool due to its cost, complexity and lack of standardization. Recent improvements in sample preparation and bioinformatics pipelines and decrease in cost now allow actionable diagnostics in patients. The potency and limits of WG-NGS and possible future indications are discussed here. WG-NGS will likely soon become a standard procedure in microbiological diagnosis.

https://www.ncbi.nlm.nih.gov/pubmed/26548640