Link to Pubmed [PMID] – 31957253
Cell. Microbiol. 2020 Jan;:e13166
Strategies employed by pathogenic enteric bacteria, such as Shigella, to subvert the host adaptive immunity are not well defined. Impairment of T lymphocyte chemotaxis by blockage of polarized edge formation has been reported upon Shigella infection. However, the functional impact of Shigella on T lymphocytes remains to be determined. Here, we show that Shigella modulates CD4+ T cell F-actin dynamics and increases cell cortical stiffness. The scanning ability of T lymphocytes when encountering antigen-presenting cells (APC) is subsequently impaired resulting in decreased cell-cell contacts (or conjugates) between the two cell types, as compared to non-infected T cells. In addition, the few conjugates established between the invaded T cells and APCs display no polarized delivery and accumulation of the TCR to the contact zone characterizing canonical immunological synapses. This is most likely due to the targeting of intracellular vesicular trafficking by the bacterial T3SS effectors IpaJ and VirA. Collective impact of these cellular reshapings by Shigella eventually results in T cell activation dampening. Altogether, these results highlight the combined action of T3SS effectors leading to T cell defects upon Shigella infection. This article is protected by copyright. All rights reserved.
https://www.ncbi.nlm.nih.gov/pubmed/31957253