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© Research
Publication : PloS one

Reconstructed ancestral sequences improve pathogen identification using resequencing DNA microarrays

Team: Archived: Group: Sylvain Brisse
Team: Microbial Evolutionary Genomics
Team: Environment and Infectious Risks
Member: Sylvain Brisse
Member: Jean-Claude Manuguerra
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in PloS one - 20 Dec 2010

Berthet N, Deletoile A, Passet V, Kennedy GC, Manuguerra JC, Cole ST, Brisse S

Link to Pubmed [PMID] – 21187950

PLoS ONE 2010;5(12):e15243

We describe the benefit of using reconstructed ancestral sequences (RAS) on resequencing microarrays for rapid pathogen identification, with Enterobacteriaceae rpoB sequences as a model. Our results demonstrate a sharp improvement of call rate and accuracy when using RASs as compared to extant sequences. This improvement was attributed to the lower sequence divergence of RASs, which also expanded the sequence space covered by the microarray. Extension of this novel microarray design strategy to viruses, antimicrobial resistance elements or toxins is straightforward.