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© Institut Pasteur
Structure de macromolécules : dimère d'aquométhémoglobine de cheval. Dérivé toxique oxydé de l'hémoglobine, représentant 1 à 2% du total.
Publication : Microbial cell factories

Quality assessment and optimization of purified protein samples: why and how?

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Microbial cell factories - 30 Dec 2014

Raynal B, Lenormand P, Baron B, Hoos S, England P

Link to Pubmed [PMID] – 25547134

Microb. Cell Fact. 2014 Dec;13:180

Purified protein quality control is the final and critical check-point of any protein production process. Unfortunately, it is too often overlooked and performed hastily, resulting in irreproducible and misleading observations in downstream applications. In this review, we aim at proposing a simple-to-follow workflow based on an ensemble of widely available physico-chemical technologies, to assess sequentially the essential properties of any protein sample: purity and integrity, homogeneity and activity. Approaches are then suggested to optimize the homogeneity, time-stability and storage conditions of purified protein preparations, as well as methods to rapidly evaluate their reproducibility and lot-to-lot consistency.

https://www.ncbi.nlm.nih.gov/pubmed/25547134