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© Research
Publication : Yeast (Chichester, England)

Purification of G1 daughter cells from different Saccharomycetes species through an optimized centrifugal elutriation procedure

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Yeast (Chichester, England) - 26 Mar 2014

Marbouty M, Ermont C, Dujon B, Richard GF, Koszul R

Link to Pubmed [PMID] – 24604765

Yeast 2014 May;31(5):159-66

Centrifugal elutriation discriminates cells according to their sedimentation coefficients, generating homogeneous samples well suited for genomic comparative approaches. It can, for instance, isolate G1 daughter cells from a Saccharomyces cerevisiae unsynchronized population, alleviating ageing and cell-cycle biases when conducting genome-wide/single-cell studies. The present report describes a straightforward and robust procedure to determine whether a cell population of virtually any yeast species can be efficiently elutriated, while offering solutions to optimize success. This approach was used to characterize elutriation parameters and S-phase progression of four yeast species (S. cerevisiae, Candida glabrata, Lachancea kluyveri and Pichia sorbitophila) and could theoretically be applied to any culture of single, individual cells.