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© Institut Pasteur/Antoinette Ryter
Coupe de Mycobacterium bovis ou bacille de Calmette et Guérin (BCG). Souche atténuée de bacille vivant, à l'origine du vaccin antituberculeux délivré par voie intradermique ou scarifications (Grossissement X 70000). Image colorisée.
Publication : Methods in molecular biology (Clifton, N.J.)

Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples.

Team: Immunobiology and Therapy
Member: Caroline Demangel
Member: Laure Guenin-Mace
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2022

Rifflet A, Demangel C, Guenin-Macé L,

Link to Pubmed [PMID] – 34643908

Link to DOI – 10.1007/978-1-0716-1779-3_13

Methods Mol Biol 2022 ; 2387(): 117-130

Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.