Link to Pubmed [PMID] – 34643908
Link to DOI – 10.1007/978-1-0716-1779-3_13
Methods Mol Biol 2022 ; 2387(): 117-130
Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.