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© Laure Mancini
Neural stem cells of the zebrafish adult telencephalon visualized by confocal microscopy
Publication : Development (Cambridge, England)

Large-scale live imaging of adult neural stem cells in their endogenous niche

Team: Zebrafish Neurogenetics
Member: Laure Bally-Cuif
Member: Nicolas Dray
Member: Sébastien Bedu
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Development (Cambridge, England) - 22 Sep 2015

Dray N, Bedu S, Vuillemin N, Alunni A, Coolen M, Krecsmarik M, Supatto W, Beaurepaire E, Bally-Cuif L

Link to Pubmed [PMID] – 26395477

Development 2015 Oct;142(20):3592-600

Live imaging of adult neural stem cells (aNSCs) in vivo is a technical challenge in the vertebrate brain. Here, we achieve long-term imaging of the adult zebrafish telencephalic neurogenic niche and track a population of >1000 aNSCs over weeks, by taking advantage of fish transparency at near-infrared wavelengths and of intrinsic multiphoton landmarks. This methodology enables us to describe the frequency, distribution and modes of aNSCs divisions across the entire germinal zone of the adult pallium, and to highlight regional differences in these parameters.