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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Methods in molecular biology (Clifton, N.J.)

Internalization assays for Listeria monocytogenes

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2014

Kühbacher A, Cossart P, Pizarro-Cerdá J

Link to Pubmed [PMID] – 24792557

Methods Mol. Biol. 2014;1157:167-78

Listeria monocytogenes is a model intracellular pathogen that can invade the cytoplasm of host mammalian cells. Cellular invasion can be measured using standard techniques such as the classical gentamicin protection assay, based on the quantification of colony-forming units from lysates of infected cells. In addition, there are methods based on immunofluorescence microscopy which allow for assaying invasion in a medium- to high-throughput manner. In the following sections we detail two different assays that can be used alone or in combination to quantify the internalization of L. monocytogenes in host cells.