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© Research
Publication : Genome biology

instaGRAAL: chromosome-level quality scaffolding of genomes using a proximity ligation-based scaffolder.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Genome biology - 18 Jun 2020

Baudry L, Guiglielmoni N, Marie-Nelly H, Cormier A, Marbouty M, Avia K, Mie YL, Godfroy O, Sterck L, Cock JM, Zimmer C, Coelho SM, Koszul R,

Link to Pubmed [PMID] – 32552806

Link to DOI – 10.1186/s13059-020-02041-z

Genome Biol 2020 06; 21(1): 148

Hi-C exploits contact frequencies between pairs of loci to bridge and order contigs during genome assembly, resulting in chromosome-level assemblies. Because few robust programs are available for this type of data, we developed instaGRAAL, a complete overhaul of the GRAAL program, which has adapted the latter to allow efficient assembly of large genomes. instaGRAAL features a number of improvements over GRAAL, including a modular correction approach that optionally integrates independent data. We validate the program using data for two brown algae, and human, to generate near-complete assemblies with minimal human intervention.