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© Carmen Buchrieser, Marie-Christine Prevost
Legionella pneumophila et son flagelle, bactérie responsable de pneumopathie aigue grave. Bactérie de l'environnement , l'émergence récente de cette maladie s'explique par son affinité pour les systèmes modernes d'alimentation en eau comme les tours de refroidissement. Image colorisée.
Publication : STAR protocols

High-content assay to measure mitochondrial function and bacterial vacuole size in infected human primary macrophages.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in STAR protocols - 17 Mar 2023

Dramé M, Garcia-Rodriguez FJ, Buchrieser C, Escoll P,

Link to Pubmed [PMID] – 36933221

Link to DOI – 10217510.1016/j.xpro.2023.102175S2666-1667(23)00133-8

STAR Protoc 2023 Mar; 4(2): 102175

Regulation of bioenergetics and cell death are pivotal mitochondrial functions determining the responses of macrophages to infection. Here, we provide a protocol to investigate mitochondrial functions during infection of macrophages by intracellular bacteria. We describe steps for quantifying mitochondrial polarization, cell death, and bacterial infection in infected, living, human primary macrophages at the single-cell level. We also detail the use of the pathogen Legionella pneumophila as model. This protocol can be adapted to investigate mitochondrial functions in other settings. For complete details on the use and execution of this protocol, please refer to Escoll et al. (2021).1.