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© Institut Pasteur
Cristaux de cellulase, enzyme purifiée de Clostridium thermocellum permettant la digestion de la cellulose. Image colorisée.
Publication : Organic & biomolecular chemistry

Gram-scale synthesis of luciferins derived from coelenterazine and original insights into their bioluminescence properties.

Team: Group : Medicinal Chemistry
Team: Diagnostic Test Innovation and Development Core Facility
Member: Yves Janin
Member: Glwadys Gagnot
Member: Vincent Hervin
Member: Racha Baatallah
Member: Thierry Rose
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Organic & biomolecular chemistry - 10 Apr 2019

Coutant EP, Goyard S, Hervin V, Gagnot G, Baatallah R, Jacob Y, Rose T, Janin YL,

Link to Pubmed [PMID] – 30882838

Link to DOI – 10.1039/c9ob00459a

Org Biomol Chem 2019 04; 17(15): 3709-3713

An original gram-scale synthesis of O-acetylated forms of coelenterazine, furimazine or hydroxy-bearing analogues of luciferins is described. The comparison over two hours of their bioluminescence, using the nanoKAZ/NanoLuc luciferase, provides remarkable insights useful for the selection of a substrate adapted for a given application.

https://pubmed.ncbi.nlm.nih.gov/30882838