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© Research
Publication : PloS one

Generation of human antigen-specific monoclonal IgM antibodies using vaccinated “human immune system” mice

Team: Innate Immunity
Member: James Di Santo
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in PloS one - 04 Oct 2010

Becker PD, Legrand N, van Geelen CM, Noerder M, Huntington ND, Lim A, Yasuda E, Diehl SA, Scheeren FA, Ott M, Weijer K, Wedemeyer H, Di Santo JP, Beaumont T, Guzman CA, Spits H

Link to Pubmed [PMID] – 20957227

PLoS ONE 2010;5(10)

Passive transfer of antibodies not only provides immediate short-term protection against disease, but also can be exploited as a therapeutic tool. However, the ‘humanization’ of murine monoclonal antibodies (mAbs) is a time-consuming and expensive process that has the inherent drawback of potentially altering antigenic specificity and/or affinity. The immortalization of human B cells represents an alternative for obtaining human mAbs, but relies on the availability of biological samples from vaccinated individuals or convalescent patients. In this work we describe a novel approach to generate fully human mAbs by combining a humanized mouse model with a new B cell immortalization technique.

http://www.ncbi.nlm.nih.gov/pubmed/20957227