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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Infection and immunity

Expression in Escherichia coli and sequence analysis of the listeriolysin O determinant of Listeria monocytogenes

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Infection and immunity - 01 Apr 1988

Mengaud J, Vicente MF, Chenevert J, Pereira JM, Geoffroy C, Gicquel-Sanzey B, Baquero F, Perez-Diaz JC, Cossart P

Link to Pubmed [PMID] – 3126142

Infect. Immun. 1988 Apr;56(4):766-72

To evaluate the role of hemolysin production in the virulence of Listeria monocytogenes, we have undertaken the analysis of the chromosomal region containing hlyA, the gene coding for listeriolysin O. A recombinant cosmid, conferring a hemolytic phenotype to Escherichia coli, was shown to express listeriolysin O, by immunoblotting with a specific antiserum against listeriolysin O. The presence of hlyA on the cosmid was demonstrated by DNA hybridization with a probe previously shown to contain part of hlyA. The complete nucleotide sequence of hlyA has been determined. The deduced protein sequence reveals the presence of a putative 25-amino-acid signal sequence: the secreted form of listeriolysin O would have 504 amino acids, in agreement with the molecular weight of purified listeriolysin O (58,000). The protein sequence is highly homologous to those of streptolysin O and pneumolysin. A peptide of 11 amino acids conserved in the three proteins contains the unique cysteine known to be essential for lytic activity. By DNA-DNA hybridization, the listeriolysin O gene was detected in all L. monocytogenes strains tested, even in the nonhemolytic type strain. The gene was absent in other species of the genus Listeria.

http://www.ncbi.nlm.nih.gov/pubmed/3126142