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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Trends in parasitology

CRISPR in Parasitology: Not Exactly Cut and Dried!

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Trends in parasitology - 18 Apr 2019

Bryant JM, Baumgarten S, Glover L, Hutchinson S, Rachidi N

Link to Pubmed [PMID] – 31006600

Trends Parasitol. 2019 Jun;35(6):409-422

CRISPR/Cas9 technology has been developing rapidly in the field of parasitology, allowing for the dissection of molecular processes with unprecedented efficiency. Optimization and implementation of a new technology like CRISPR, especially in nonmodel organisms, requires communication and collaboration throughout the field. Recently, a ‘CRISPR in Parasitology’ symposium was held at the Institut Pasteur Paris, bringing together scientists studying Leishmania, Plasmodium, Trypanosoma, and Anopheles. Here we share technological advances and challenges in using CRISPR/Cas9 in the parasite and vector systems that were discussed. As CRISPR/Cas9 continues to be applied to diverse parasite systems, the community should now focus on improvement and standardization of the technique as well as expanding the CRISPR toolkit to include Cas9 alternatives/derivatives for more advanced applications like genome-wide functional screens.

https://www.ncbi.nlm.nih.gov/pubmed/31006600