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© Research
Publication : Eukaryome Impact on Human Intestine Homeostasis and Mucosal Immunology

A Protocol to Quantify Cellular Morphodynamics: From Cell Labelling to Automatic Image Analysis

Team: Biological Image Analysis
Member: Maria Manich
Member: Aleix Boquet-Pujadas
Member: Nancy Guillen
Member: Jean-Christophe Olivo-Marin
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Eukaryome Impact on Human Intestine Homeostasis and Mucosal Immunology - 04 Jun 2020

Maria Manich, Aleix Boquet-Pujadas, Stéphane Dallongeville, Nancy Guillen, Jean-Christophe Olivo-Marin

Link to DOI – 10.1007/978-3-030-44826-4_25

Eukaryome Impact on Human Intestine Homeostasis and Mucosal Immunology, 2020

Abstract
Cellular morphodynamics can be used as markers for many physiological and pathological processes. This protocol provides a step-by-step guide to identify variations in motility and morphology within (or across) cell populations using non-invasive live imaging and reproducible image analysis techniques such as segmentation and tracking. Detailed instructions cover all the way from cell culturing and labelling to automatic image and statistical analyses, including the definition of multiple descriptors that characterise the shape and movement of cells in a quantitative manner. All methods are available as free open-source software and illustrated by video tutorials.

Keywords
Cell biology Image analysis Software Segmentation Tracking Shape descriptors