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© Research
Publication : Methods in molecular biology (Clifton, N.J.)

A luciferase reporter for gene expression studies and dynamic imaging of superficial Candida albicans infections

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2012

Pietrella D, Enjalbert B, Zeidler U, Znaidi S, Rachini A, Vecchiarelli A, d'Enfert C

Link to Pubmed [PMID] – 22328402

Methods Mol. Biol. 2012;845:537-46

Real-time imaging of fungal infections is becoming integral to the study of host-pathogen interactions, as it allows monitoring of the spatial and temporal progression of pathogen growth or of the host response in a single animal as well as reducing the number of animals used to obtain significant data. We present different applications of a novel luciferase reporter gene constructed from the coding sequences of the Candida albicans PGA59 gene, encoding a GPI-linked cell wall protein, and the Gaussia princeps luciferase gene. Upon addition of the coelenterazine substrate, light produced by the surface-exposed luciferase can be used to quantify gene expression from a variety of C. albicans promoters as well as monitoring cutaneous, subcutaneous, and vaginal infections.

http://www.ncbi.nlm.nih.gov/pubmed/22328402