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© Research
Publication : Biomedical Imaging: From Nano to Macro, 2009. ISBI

A compressed sensing approach for biological microscopic image processing

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Biomedical Imaging: From Nano to Macro, 2009. ISBI - 01 Jul 2009

Marcio Marim, Elsa Angelini and Jean-Christophe Olivo-Marin

Biomedical Imaging: From Nano to Macro, 2009. ISBI '09. IEEE International Symposium on

In fluorescence microscopy the noise level and the photobleaching are cross-dependent problems since reducing exposure time to reduce photobleaching degrades image quality while increasing noise level. These two problems cannot be solved independently as a post-processing task, hence the most important contribution in this work is to a-priori denoise and reduce photobleaching simultaneously by using the compressed sensing framework (CS). In this paper, we propose a CS-based denoising framework, based on statistical properties of the CS optimality, noise reconstruction characteristics and signal modeling applied to microscopy images with low signal-to-noise ratio (SNR). Our approach has several advantages over traditional denoising methods, since it can under-sample, recover and denoise images simultaneously. We demonstrate with simulated and practical experiments on fluorescence image data that thanks to CS denoising we can obtain images with similar or increased SNR while still being able to reduce exposition times.