The SRC Kinase Adaptor Phosphoprotein 2 (SKAP2), a broadly expressed protein recruiting protein partners to specific subcellular domains, plays a central role in multiple processes including TGFb response, integrin signalling, and cell migration. It also controls susceptibility to autoimmune and infectious diseases and metastatic progression.
The goal of this project is to better understand SKAP2 functions by using two complementary approaches: a high-throughput yeast two-hybrid screening validated by a luciferase complementation assay coupled with site-directed mutagenesis in human cells. We will further analyse the fine-tuning between the binding of SRC kinases to SKAP2 dimers and their activations, precise binding sites of partners, and compare the SKAP2 interactome with that of its SKAP1 paralog. The knowledge of the pathophysiological pathways in which susceptibility loci such SKAP2 act is necessary to understand human complex diseases and develop new strategy of treatments.