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  • Director of Department
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© Institut Pasteur
Macrophages et lymphocytes de souris. Image colorisée.
Scientific Fields
Diseases
Organisms
Applications
Technique

Projects

CV

Birth date : June 4, 1981
Nationality: Lithuanian

LANGUAGE SKILLS :
Fluent in English, Russian Lithuanian and French

WORK EXPERIENCE :

  • 2014 – current : Lymphopoiesis unit, Inserm U668, Institut Pasteur, Paris, France. Post doctoral position
  • 2010 – 2014 : National Institute of Health and Medical Research (INSERM) U1020, Hôpital Broussais, Faculty of Medicine, Differentiation and Physiology of T Lymphocytes laboratory, Paris, France. Post doctoral position
  • 2009, three months : The National Institute for Agricultural Research (INRA) The Microbiology and Food Chain Division (MICA), Jouy en Josas, France. Writing a scientific and management reports to European Commission on Marie Curie LabHealth programme
  • 2006 – 2009 : The National Institute for Agricultural Research (INRA) Department of Microbial Genetics, Jouy en Josas, France. Marie Curie early stage researcher PhD grant, LabHealth programme. “An in silico approach to the identification of proteins involved in bacteria-host interactions: a case-study of Lactobacillus delbrueckii ssp. bulgaricus and related lactobacilli”
  • 2003 – 2005 : Institute of Biotechnology, Prokaryote Gene Engineering Laboratory, Vilnius, Lithuania. “Use of the methylation activity-based selection for altering sequence specificity of the Eco57ID78K/D899E restriction endonuclease mutant”
  • 2001 – 2003 : Vilnius University Institute of Oncology, Vilnius, Lithuania. “Identification of oncogenic human papillomavirus types and studying HPV16 E6 gene polymorphism in cervical cancerogenesis”

SCIENTIFIC SKILLS :

  • Cellular biology : Cell cultures, dissection and construction of chimeric mice, cell suspensions from organs, purification of cells by depletion, adoptive T cell transfers. Immunofluorescence labeling, flow cytometry and data analysis using FACSDiva and FlowJo
  • Microscopy : Organ and tissue cryosectioning techniques, immunostaining. in vivo time-lapse two- photon microscope imaging, fluorescence and confocal microscopy methods. Image analysis using Imaris and ImageJ software
  • Molecular biology : DNA/RNA manipulation techniques, primer design, PCR methods, DNA cloning and bacterial transformation procedures, heterologous protein expression procedures
  • Bioinformatics DNA and protein sequence analysis: Blast, ClustalW, HMMER. Protein localization, signal peptide predictions. Conserved domain and pattern searches: InterPro, Pfam, Superfamily. Phylogenetic networks. ImageJ macro writing

HIGHER EDUCATION :

2006 – 2009 : PhD. Université Paris XI Orsay & INRА, Department of Microbial Genetics, Jouy en Josas, France.

2003 – 2005 : Master of Science. Main subject of studies – Genetics. Vilnius University, Lithuania

1999 – 2003 : Bachelor degree. Main subject of studies – Molecular Biology. Vilnius University, Lithuania

PUBLICATIONS :

Barinov A, Loux V, Hammani A, Nicolas P, Langella P, Ehrlich D, Maguin E, van de Guchte M. Prediction of surface exposed proteins in Streptococcus pyogenes, with a potential application to other Gram-positive bacteria. Proteomics. 2009 Jan; 9(1):61-73

BOOK CHAPTER :

Barinov A, Bolotine A, Langella P, Maguin E, van de Guchte M. Genomics of the genus Lactobacillus in “Lactic Acid Bacteria and Bifidobacteria: Current Progress in Advanced Research” Caister Academic Press, Editor: Kenji Sonomoto and Atsushi Yokota, ISBN: 978-1-904455-82-0

PATENT :

Barinov A, Pichugin A, Vassetzky Y. Method for selecting nucleic acid molecules with a desired nucleotide sequence. Pending, European provisional Nr. EP12305954.5; US provisional Nr. 61/678.652. Aug. 2012

SELECTED PRESENTATION :

Novel approach for the prediction of surface exposed proteins from Gram-positive bacteria. 15th Meeting of the Club des Bactéries Lactiques, 13-15 November 2007, Rennes, France

Publications

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