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Work in Progress – GENOMES and GENETICS seminars “Acquisition of antibiotic resistance favors the prepared E. coli ” and “Beyond antibiograms: a model-based approach to characterize enzyme-mediated resistance in bacteria “

WIP – 1/ Ana PEREZ COBAS “Analysis of the human lung microbiome during pneumonia and antibiotic therapy” 2/ David PEREZ PASCUAL “Identification of key microbiota members providing resistance to infection in a gnotobiotic zebrafish model”

© Research

Event

“Unveiling the small RNA-target interaction networks of Staphylococcus aureus” Genomes and Genetics and Microbiology departments joint seminar

Department of: Genomes and Genetics

Department of: Microbiology

Member: Micheline Fromont-Racine

Scientific Fields

Diseases

Organisms

Applications

Technique

Date

27

Feb 2020

Time

11:00:00

25-28 Rue du Dr Roux, 75015 Paris, France

Address

Building: rdc Centre François Jacob Room: auditorium du CFJ

Location

2020-02-27 11:00:00 2020-02-27 12:30:00 Europe/Paris “Unveiling the small RNA-target interaction networks of Staphylococcus aureus” Genomes and Genetics and Microbiology departments joint seminar Abstract: It is now well recognized that RNAs are key intracellular effectors in fast adaptive processes. In many cases, they co-regulate the expression of functionally related genes at the post-transcriptional level. Today, more than […] 25-28 Rue du Dr Roux, 75015 Paris, France Micheline Fromont-Racine micheline.fromont-racine@pasteur.fr

Speakers

Pascale ROMBY

(Université de Strasbourg, CNRS, IBMC, Strasbourg)

About

Abstract:

It is now well recognized that RNAs are key intracellular effectors in fast adaptive processes. In many cases, they co-regulate the expression of functionally related genes at the post-transcriptional level. Today, more than 200 potentially regulatory RNAs have been identified so far in S. aureus. In order to define their direct targets and the regulatory networks in which they belong, we have recently applied the MS2-affinity purification approach coupled with RNA sequencing to several non coding RNAs in S. aureus (1,2). Using a combination of in vivo and in vitro approaches, numerous of the co-purified and enriched mRNAs have been validated as direct targets. Several non coding RNAs will be used to illustrate the intricate interactions between RNAs, two-component systems and transcriptional regulatory proteins and their implication in stress responses (including oxidative and NO), sugar metabolism and virulence.

(1) Bronesky, Desgranges et al. (2019) A multifaceted small RNA modulates gene expression upon glucose limitation in Staphylococcus aureus. EMBO J. Mar 15; 38(6). (2) Tomasini et al. (2017) The RNA targetome of Staphylococcus aureus non-coding RNA RsaA: impact on cell surface properties and defense mechanisms. Nucleic Acids Res. 45(11):674

by: Pascale ROMBY Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC, 67084 Strasbourg

Partners

Location

Building: rdc Centre François Jacob
Room: auditorium du CFJ
Address: 25-28 Rue du Dr Roux, 75015 Paris, France

Published on: 10 Feb 2020

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