It is now well recognized that RNAs are key intracellular effectors in fast adaptive processes. In many cases, they co-regulate the expression of functionally related genes at the post-transcriptional level. Today, more than 200 potentially regulatory RNAs have been identified so far in S. aureus. In order to define their direct targets and the regulatory networks in which they belong, we have recently applied the MS2-affinity purification approach coupled with RNA sequencing to several non coding RNAs in S. aureus (1,2). Using a combination of in vivo and in vitro approaches, numerous of the co-purified and enriched mRNAs have been validated as direct targets. Several non coding RNAs will be used to illustrate the intricate interactions between RNAs, two-component systems and transcriptional regulatory proteins and their implication in stress responses (including oxidative and NO), sugar metabolism and virulence.
(1) Bronesky, Desgranges et al. (2019) A multifaceted small RNA modulates gene expression upon glucose limitation in Staphylococcus aureus. EMBO J. Mar 15; 38(6). (2) Tomasini et al. (2017) The RNA targetome of Staphylococcus aureus non-coding RNA RsaA: impact on cell surface properties and defense mechanisms. Nucleic Acids Res. 45(11):674
by: Pascale ROMBY Architecture et Réactivité de l’ARN, Université de Strasbourg, CNRS, IBMC, 67084 Strasbourg
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