The team is working in the RNA degradation pathways in Eukaryotes using the yeast, Saccharomyces cerevisiae, as an eukaryotic model. We are interested in the cytoplasmic general mRNA degradation pathway and in the elimination of aberrant or non-conform mRNAs such as those lacking a Stop codon (Non-Stop mRNA). These events lead to truncated peptides, which may have toxic effects. Cytoplasmic quality control processes recognize ribosomes that are stalled during translating and selectively eliminate the corresponding aberrant mRNAs as well as the aberrant polypeptides to maintain protein homeostasis. We recently identified the RQC complex, which recognizes the aberrant peptides generated from Non-Stop mRNAs and target them to degradation by the proteasome (Defenouillère et al., PNAS 2013; JBC. 2016; Mol. Biol. Cell. 2016; Curr. Genet. 2016).
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2020A structural inventory of native ribosomal ABCE1-43S pre-initiation complexes., EMBO J 2021 01; 40(1): e105179.
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2019A specialised SKI complex assists the cytoplasmic RNA exosome in the absence of direct association with ribosomes, EMBO J. 2019 07;38(14):e100640.
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2019Structure of the 80S ribosome-Xrn1 nuclease complex, Nat. Struct. Mol. Biol. 2019 04;26(4):275-280.
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2017The ribosome-bound quality control complex: from aberrant peptide clearance to proteostasis maintenance, Curr. Genet. 2017 Dec;63(6):997-1005.
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2017The ribosome-bound quality control complex remains associated to aberrant peptides during their proteasomal targeting and interacts with Tom1 to limit protein aggregation, Mol. Biol. Cell 2017 May;28(9):1165-1176.
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2016The cryo-EM structure of a ribosome-Ski2-Ski3-Ski8 helicase complex, Science 2016 12;354(6318):1431-1433.
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2016Rqc1 and Ltn1 Prevent C-terminal Alanine-Threonine Tail (CAT-tail)-induced Protein Aggregation by Efficient Recruitment of Cdc48 on Stalled 60S Subunits, J. Biol. Chem. 2016 Jun;291(23):12245-53.
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2014mRNA Degradation and Decay (book chapter), Fungal RNA Biology.
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2013Yeast ribosomal protein L7 and its homologue Rlp7 are simultaneously present at distinct sites on pre-60S ribosomal particles, Nucleic Acids Res. 2013 Nov;41(20):9461-70.
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2013Cdc48-associated complex bound to 60S particles is required for the clearance of aberrant translation products, Proc. Natl. Acad. Sci. U.S.A. 2013 Mar;110(13):5046-51.
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