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Scientific Fields
Diseases
Organisms
Applications
Technique
Date
14
Feb 2019
Time
11:00:00
Institut Pasteur, Rue du Docteur Roux, Paris, France
Address
Building: François Jacob Room: Auditorium F.Jacob
Location
2019-02-14 11:00:00 2019-02-14 12:00:00 Europe/Paris MicroOmiX : Enabling droplet microfluidics technologies SINGLE CELL PROTEOMICS TO SINGLE CELL SECRETOMICS Droplet-based microfluidics allow the reliable production of micrometer-sized incubators in which individual cells can be isolated and manipulated at high throughput rates. Such microfluidics-based technologies have been […] Institut Pasteur, Rue du Docteur Roux, Paris, France Pierre-Henri Commere pierre-henri.commere@pasteur.fr

Speakers

Sylvain Ursuegi
(faculté de Pharmacie Illkirch )

About

SINGLE CELL PROTEOMICS TO SINGLE CELL SECRETOMICS

Droplet-based microfluidics allow the reliable production of micrometer-sized incubators in which individual cells can be isolated and manipulated at high
throughput rates. Such microfluidics-based technologies have been successfully interfaced with RNA-Seq enabling the analysis of cell heterogeneity at the
transcriptome level. However, those technologies still suffer from serious limitations linked to the lack of efficient method for in-droplet biomarker capture and purification preventing the possibility to perform multi-omic investigations (transcriptomic/proteomic).
MicroOmiX addresses these limitations by combining two proprietary technologies:

    •  a highly efficient and robust capture system based on functional surfactant
      allowing the scavenging of a wide range of biomarkers;
    • a droplet washing technology based on an inversion process enabling the
      separation of captured biomarkers from contaminants and other cell remnants

The detection of purified biomarkers can then be performed using current analyzer:

  • a cytometer for a targeted approach (up to 30 RNA/protein biomarkers) or
  • a sequencer for a non-targeted transcriptomic strategy combined with targeted proteomic.

    Download (PDF, 131KB)

Location

Building: François Jacob
Room: Auditorium F.Jacob
Address: Institut Pasteur, Rue du Docteur Roux, Paris, France